Chromatographia

, 67:893 | Cite as

Simultaneous Determination of Tamsulosin and Dutasteride in Human Plasma by LC–MS–MS

  • Sangita Agarwal
  • K. Veeran Gowda
  • Amlan Kanti Sarkar
  • Debotri Ghosh
  • Uttam Bhaumik
  • Tapas Kumar Chattaraj
  • Tapan Kumar Pal
Original

Abstract

A sensitive and selective liquid chromatographic tandem mass spectrometric (LC–MS–MS) method was developed for simultaneous identification and quantification of tamsulosin and dutasteride in human plasma, which was well applied to clinical study. The method was based on liquid–liquid extraction, followed by an LC procedure with a Gemini C-18, 50 mm × 2.0 mm (3 μm) column and using methanol:ammonium formate (97:3, v/v) as the mobile phase. Protonated ions formed by a turbo ionspray in positive mode were used to detect analytes and internal standard. MS–MS detection was by monitoring the fragmentation of 409.1 → 228.1 (m/z) for tamsulosin, 529.3 → 461.3 (m/z) for dutasteride and 373.2 → 305.3 (m/z) for finasteride (IS) on a triple quadrupole mass spectrometer. The lower limit of quantification for both tamsulosin and dutasteride was 1 ng mL−1. The proposed method enables the unambiguous identification and quantification of tamsulosin and dutasteride for clinical drug monitoring.

Keywords

Column liquid chromatography–mass spectrometry Pharmacokinetics Validation Tamsulosin and dutasteride 

Notes

Acknowledgments

The authors are thankful to DPRP/PRDSF of Department of Science and Technology (DST), New Delhi (Grant No.VII-PRDSF/56/05-06/TDT) for providing the instrumental facilities and financial support for carrying out this study and also to Burgeon Pharmaceutical Pvt. Ltd., Chennai, India for supplying the samples.

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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2008

Authors and Affiliations

  • Sangita Agarwal
    • 1
  • K. Veeran Gowda
    • 1
  • Amlan Kanti Sarkar
    • 1
  • Debotri Ghosh
    • 1
  • Uttam Bhaumik
    • 1
  • Tapas Kumar Chattaraj
    • 2
  • Tapan Kumar Pal
    • 1
  1. 1.Department of Pharmaceutical TechnologyJadavpur UniversityKolkataIndia
  2. 2.N.R.S. Medical College and HospitalKolkataIndia

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