Chromatographia

, Volume 67, Issue 9–10, pp 679–686 | Cite as

LC Determination of Malondialdehyde Concentrations in the Human Umbilical Vein Endothelial Cell Culture Medium: Application to the Antioxidant Effect of Vitexin-2″-O-rhamnoside

  • Xixiang Ying
  • Haibo Li
  • Zhili Xiong
  • Zhaoshu Sun
  • Shuang Cai
  • Wenliang Zhu
  • Yujin Bi
  • Famei Li
Original

Abstract

Vitexin-2″-O-rhamnoside (VOR) presents the leaves of Crataegus pinnatifida Bge. var. major which plays a role in preventing human pathologies related to oxidative stress, but as the principal component in the leaves, little attention has been devoted to its study of antioxidation of VOR. A simple, rapid and sensitive high-performance liquid chromatography method was developed to determine malondialdehyde (MDA) in ECV304 cell culture medium induced by tert-butyl-hydroperoxide (TBHP). The preparation of analyzed samples involved a one-step derivatization with thiobarbituric acid (TBA). The separation was achieved using the Synergi Hydro-RP, a polar endcapped C18 column (250 × 4.6 mm, 4 μm pore size) with a linear gradient elution of acetonitrile and 10 mmol L−1 of ammonium acetate aqueous solution (pH 6.8). The calibration curve was linear over the ranges 0.0125–1.25 μmol L−1 MDA (r = 0.9962). The lower limit of quantification of MDA was 0.0125 μmol L−1. Relative standard deviations of intra-day and inter-day precision were less than 5.2 and 4.4%. The method with high recovery (95.4 ± 1.3%) was successfully applied to the investigation of antioxidation of VOR by determining MDA in ECV304 cell culture medium.

Keywords

Column liquid chromatography Human umbilical vein endothelial cell Antioxidation Malondialdehyde Vitexin-2″-O-rhamnoside 

Notes

Acknowledgment

This work was supported by the funds of Natural Science Foundation of Liaoning Province (20072073) and Scientific Research Planning of the Educational Department of Liaoning Province (2004D264), China.

References

  1. 1.
    Ding X, Jiang Y, Zhong Y, Zuo C (1990) China J Chin Materal Med 15:295–297, 319Google Scholar
  2. 2.
    Ninfali P, Bacchiocca M, Antonelli A, Biagiotti E, Di Gioacchino AM, Piccoli G, Stocchi V, Brandi G (2007) Phytomedicine 14:216–221CrossRefGoogle Scholar
  3. 3.
    Ying XX, Gao S, Zhu WL, Bi YJ, Qin F, Li XQ, Li FM (2007) J Pharmaceut Biomed Anal 44:802–806CrossRefGoogle Scholar
  4. 4.
    Ma G, Jiang XH, Chen Z, Ren J, Li CR, Liu TM (2007) J Pharmaceut Biomed Anal 44:243–249CrossRefGoogle Scholar
  5. 5.
    Ying XX, Lu XM, Sun XH, Li XQ, Li FM (2007) Talanta 72:1500–1506CrossRefGoogle Scholar
  6. 6.
    Kim JH, Lee BC, Kim JH, Sim GS, Lee DH, Lee KE, Yun YP, Pyo HB (2005) Arch Pharm Res 28:195–202Google Scholar
  7. 7.
    Gadow A, Joubert E, Hansmann CF (1997) J Agric Food Chem 45:632–638CrossRefGoogle Scholar
  8. 8.
    Soszynski M, Bartosz G (1997) Free Radic Biol Med 23:463–469CrossRefGoogle Scholar
  9. 9.
    Gorbunov NV, Tyurina YY, Salama G, Day BW, Claycamp HG, Argyros G, Elsayed NM, Kagan VE (1998) Biochem Biophys Res Commun 244:647–651CrossRefGoogle Scholar
  10. 10.
    Kennedy CH, Church DF, Winston GW, Pryor WA (1992) Biol Med 12:381–387Google Scholar
  11. 11.
    Wade CR, Jackson PG, Van Rij AM (1985) Biochem Med 33:291–296CrossRefGoogle Scholar
  12. 12.
    Klvanova J, Beno I, Ondreicka R, Volkovova K, Staruchova M, Grancicova E, Bobek P, Tatara M (1998) Bratisl Lek Listy 99:245–249Google Scholar
  13. 13.
    Draper HH, Squires EJ, Mahmoodi H, Wu J, Agarwal S, Hadley M (1993) Free Radic Biol Med 15:353–363CrossRefGoogle Scholar
  14. 14.
    Janero DR (1990) Free Radic Biol Med 9:515–540CrossRefGoogle Scholar
  15. 15.
    Davey MW, Stals E, Panis B, Keulemans J, Swennen RL (2005) Anal Biochem 347:201–207CrossRefGoogle Scholar
  16. 16.
    Mateos R, Lecumberri E, Ramos S, Goya L, Bravo L (2005) J Chromatogr B Analyt Technol Biomed Life Sci 827:76–82CrossRefGoogle Scholar
  17. 17.
    Suttnar J, Masova L, Dyr JE (2001) J Chromatogr B Biomed Sci Appl 751:193–197CrossRefGoogle Scholar
  18. 18.
    Roman K, Vladimira M, Alexander C (2002) Clin Chem Lab Med 40:1032–1035CrossRefGoogle Scholar
  19. 19.
    Agarwal R, Chase SD (2002) J Chromatogr B Analyt Technol Biomed Life Sci 775:121–126CrossRefGoogle Scholar
  20. 20.
    Hermans N, Cos P, Berghe DV, Vlietinck AJ, de Bruyne T (2005) J Chromatogr B Analyt Technol Biomed Life Sci 822:33–39CrossRefGoogle Scholar
  21. 21.
    Grotto D, Santa Maria LD, Boeira S, Valentini J, Charão MF, Moro AM, Nascimento PC, Pomblum VJ, Garcia SC (2007) J Pharm Biomed Anal 43:619–624CrossRefGoogle Scholar
  22. 22.
    Seljeskog E, Hervig T, Mansoor MA (2006) Clin Biochem 39:947–954CrossRefGoogle Scholar
  23. 23.
    Sun JY, Yang SB, Song HX, Li GH, Qiu HX (2002) Chin Tradit Herbal Drugs 6:483–486Google Scholar
  24. 24.
    Daniel J, Antolovich M, Prenzler PD, Robards K (2002) J Agric Food Chem 50:1720–1724CrossRefGoogle Scholar
  25. 25.
    Beyer G, Melzig MF (2005) Biol Pharm Bull 28:1183–1186CrossRefGoogle Scholar
  26. 26.
    Mosmann J (1983) J Immunol 65:55–56CrossRefGoogle Scholar
  27. 27.
    Dumont P, Burton M, Chen QM, Gonos ES, Frippiat C, Mazarati JB, Eliaers F, Remacle J, Toussaint O (2000) Free Radic Biol Med 28:361–373CrossRefGoogle Scholar
  28. 28.
    Korchazhkina O, Exley C, Andrew Spencer S (2003) J Chromatogr B Analyt Technol Biomed Life Sci 794:353–362CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2008

Authors and Affiliations

  • Xixiang Ying
    • 1
    • 2
  • Haibo Li
    • 3
  • Zhili Xiong
    • 1
  • Zhaoshu Sun
    • 2
  • Shuang Cai
    • 1
  • Wenliang Zhu
    • 1
  • Yujin Bi
    • 1
  • Famei Li
    • 1
  1. 1.School of PharmacyShenyang Pharmaceutical UniversityShenyangChina
  2. 2.School of PharmacyLiaoning University of Traditional Chinese MedicineShenyangChina
  3. 3.School of Basic Medical SciencesLiaoning University of Traditional Chinese MedicineShenyangChina

Personalised recommendations