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Chromatographia

, Volume 67, Issue 9–10, pp 767–772 | Cite as

Dynamic On-Column Eluent Modification: A Novel Strategy for Peak Resolution Enhancement. Application to the Preparative Separation of Ecdysteroid Isomers

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Abstract

A novel LC method was applied to enhance the peak resolution of two ecdysteroids: 20-hydroxyecdysone and its 3-epimer. An isocratic solvent system of methanol–water (6:4, v/v) was used on a C18 column, and 100 μL of water was injected during the development in such a manner that the eluted solvent peak appeared exactly between the two overlapping peaks. This resulted in the increased retardation of the later-eluted peak, and in a good separation of the two compounds within 4.5 min.

Keywords

Column liquid chromatography Preparative LC Ecdysteroid 20-Hydroxyecdysone and 3-epi-20-hydroxyecdysone 

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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2008

Authors and Affiliations

  1. 1.Department of PharmacognosyUniversity of SzegedSzegedHungary
  2. 2.Department of Pharmacology and PharmacotherapySemmelweis UniversityBudapestHungary

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