Abstract
A novel LC method was applied to enhance the peak resolution of two ecdysteroids: 20-hydroxyecdysone and its 3-epimer. An isocratic solvent system of methanol–water (6:4, v/v) was used on a C18 column, and 100 μL of water was injected during the development in such a manner that the eluted solvent peak appeared exactly between the two overlapping peaks. This resulted in the increased retardation of the later-eluted peak, and in a good separation of the two compounds within 4.5 min.
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Hunyadi, A., Kalász, H. & Báthori, M. Dynamic On-Column Eluent Modification: A Novel Strategy for Peak Resolution Enhancement. Application to the Preparative Separation of Ecdysteroid Isomers. Chroma 67, 767–772 (2008). https://doi.org/10.1365/s10337-008-0557-y
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DOI: https://doi.org/10.1365/s10337-008-0557-y