, Volume 67, Issue 7–8, pp 627–631 | Cite as

Determination and Pharmacokinetic Study of Calycosin-7-O-β-d-glucoside in Rat Plasma After Intravenous Administration of Aidi Lyophilizer

  • Qing Li
  • Liang Xu
  • Ting-Ting Wang
  • Ying Jia
  • Zhi-Wei Wang
  • Kai-Shun Bi
Full Short Communication


Aidi injection is a clinical medicine used in China for the treatment of cancer. Calycosin-7-O-β-d-glucoside is the main effective components of the formulas. In this study, a high performance liquid chromatographic (LC) method was developed to quantify calycosin-7-O-β-d-glucoside in rat plasma using a liquid–liquid extraction and ultraviolet (UV) absorbance detection. LC analysis was performed on a Diamonsil C18 column (200 × 4.6 mm i.d., 5 μm particle size) with isocratic mobile phase consisting of acetonitrile–0.05% phosphoric acid (19.5:80.5, v/v) of a flow rate of 1.0 mL min−1. The linear range was 0.11–17.6 μg mL−1 and the low quantification limit was 0.11 μg mL−1 (S/N = 10). The intra- and inter-day relative standard deviations (RSD) in the measurement of quality control (QC) samples 0.11, 0.22, 1.32 and 8.80 μg mL−1 ranged from 4.1 to 6.3 and 4.3 to 6.2%, respectively. The accuracy was from −6.7 to 4.3% in terms of relative error (RE). Calycosin-7-O-β-d-glucoside was stable in storage at −20 °C for 2 weeks and stable after three freeze–thaw cycles in rat plasma. This method was validated for specificity, accuracy, precision and was successfully applied to pharmacokinetic study of calycosin-7-O-β-d-glucoside in rat plasma after intravenous administration of Aidi lyophilizer.


Column liquid chromatography Pharmacokinetic study Aidi lyophilizer Rat plasma Calycosin-7-O-β-d-glucoside 


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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2008

Authors and Affiliations

  • Qing Li
    • 1
  • Liang Xu
    • 1
    • 2
  • Ting-Ting Wang
    • 1
  • Ying Jia
    • 1
  • Zhi-Wei Wang
    • 1
  • Kai-Shun Bi
    • 1
  1. 1.School of PharmacyShenyang Pharmaceutical UniversityLiaoning ProvincePeople’s Republic of China
  2. 2.The Analysis and Testing CenterInner Mongolia University for NationalitiesInner Mongolia ProvincePeople’s Republic of China

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