Separation of Sesquiterpenes from Yarrow (Achillea millefolium L. s. l.) by LC-MS on Non-Porous Columns
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Species of the genus Achillea have been used in traditional folk medicine for centuries. In Europe taxa of the Achillea millefolium group are widely spread, their correct taxonomic differentiation by morphological and anatomical characteristics encounters some difficulties. Several species of the polyploid A. millefolium group however, can be characterised by their distinct sesquiterpene pattern. Analysis is usually performed by LC using conventional RP stationary phases. Likewise, it has been proven that non-porous RP stationary phases are an excellent alternative for sensitive and more rapid separations of plant extracts. In the present work a Kovasil MS-C18 1.5 μm non-porous packing was used with an acetonitrile-water gradient for the analysis of Achillea flower extracts. Detection and identification of the respective sesquiterpenes has been achieved by diode array detection and LC coupled APCI+ and ESI+ mass spectrometry.
KeywordsColumn liquid chromatography–mass spectrometry Non-porous stationary phases Sesquiterpene Achillea millefolium
This work was supported in by the grant KM1 046476 from the Hungarian National Science Foundation (OTKA).
- 2.Wichtl M (2002) Teedrogen 4th edition.Wiss. Verlags GesmbH, StuttgartGoogle Scholar
- 3.Rauchensteiner F, Nejati S, Werner I, Glasl S, Saukel J, Jurenitsch J, Kubelka W (2002) Sci Pharm 70:199–230Google Scholar
- 4.Seaman FC (1982) Bot Rev 48:415–416Google Scholar
- 7.Mustakerova E, Todorova M, Tsankova E (2002) Z Naturfosch 57:568–570Google Scholar
- 8.Simic N, Palic R, Vajs V, Milosavljevic S, Djokovic D (2002) J Essent Oil Res 14:76–78Google Scholar
- 10.Konyalioglu S, Karamenderes CJ (2007) J Ethnopharmacol 103:221–227Google Scholar
- 12.Saukel J, Länger R (1992) Phyton 31:185–207Google Scholar
- 13.Saukel J, Länger R (1992) Phyton 32:47–78Google Scholar
- 17.Rothwangl K, Glasl S, Werner I, Reznicek G (2004) Sci Pharm 72:249–258Google Scholar