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Separation of Sesquiterpenes from Yarrow (Achillea millefolium L. s. l.) by LC-MS on Non-Porous Columns

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Abstract

Species of the genus Achillea have been used in traditional folk medicine for centuries. In Europe taxa of the Achillea millefolium group are widely spread, their correct taxonomic differentiation by morphological and anatomical characteristics encounters some difficulties. Several species of the polyploid A. millefolium group however, can be characterised by their distinct sesquiterpene pattern. Analysis is usually performed by LC using conventional RP stationary phases. Likewise, it has been proven that non-porous RP stationary phases are an excellent alternative for sensitive and more rapid separations of plant extracts. In the present work a Kovasil MS-C18 1.5 μm non-porous packing was used with an acetonitrile-water gradient for the analysis of Achillea flower extracts. Detection and identification of the respective sesquiterpenes has been achieved by diode array detection and LC coupled APCI+ and ESI+ mass spectrometry.

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Acknowledgments

This work was supported in by the grant KM1 046476 from the Hungarian National Science Foundation (OTKA).

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Authors and Affiliations

  1. Department of Biochemistry and Medical Chemistry, Faculty of Medicine, University of Pecs, 12. Szigeti str, 7624, Pecs, Hungary

    Gergely Montsko, Aniko Takatsy, Robert Ohmacht & Laszlo Mark

  2. Institute of Pharmaceutical Chemistry, University of Pécs, Rókus str. 2, 7624, Pécs, Hungary

    Borbala Boros

  3. Department of Pharmacognosy, Faculty of Life Sciences, University of Vienna, Althanstraße 14, 1090, Vienna, Austria

    Sabine Glasl, Liselotte Krenn & Gottfried Reznicek

Authors
  1. Gergely Montsko
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  2. Borbala Boros
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  3. Aniko Takatsy
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  4. Robert Ohmacht
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  5. Sabine Glasl
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  6. Liselotte Krenn
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  7. Laszlo Mark
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  8. Gottfried Reznicek
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Correspondence to Robert Ohmacht.

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Montsko, G., Boros, B., Takatsy, A. et al. Separation of Sesquiterpenes from Yarrow (Achillea millefolium L. s. l.) by LC-MS on Non-Porous Columns. Chroma 67, 467–470 (2008). https://doi.org/10.1365/s10337-008-0520-y

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  • DOI: https://doi.org/10.1365/s10337-008-0520-y

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