, Volume 67, Issue 5–6, pp 467–470 | Cite as

Separation of Sesquiterpenes from Yarrow (Achillea millefolium L. s. l.) by LC-MS on Non-Porous Columns

  • Gergely Montsko
  • Borbala Boros
  • Aniko Takatsy
  • Robert Ohmacht
  • Sabine Glasl
  • Liselotte Krenn
  • Laszlo Mark
  • Gottfried Reznicek
Full Short Communication


Species of the genus Achillea have been used in traditional folk medicine for centuries. In Europe taxa of the Achillea millefolium group are widely spread, their correct taxonomic differentiation by morphological and anatomical characteristics encounters some difficulties. Several species of the polyploid A. millefolium group however, can be characterised by their distinct sesquiterpene pattern. Analysis is usually performed by LC using conventional RP stationary phases. Likewise, it has been proven that non-porous RP stationary phases are an excellent alternative for sensitive and more rapid separations of plant extracts. In the present work a Kovasil MS-C18 1.5 μm non-porous packing was used with an acetonitrile-water gradient for the analysis of Achillea flower extracts. Detection and identification of the respective sesquiterpenes has been achieved by diode array detection and LC coupled APCI+ and ESI+ mass spectrometry.


Column liquid chromatography–mass spectrometry Non-porous stationary phases Sesquiterpene Achillea millefolium 



This work was supported in by the grant KM1 046476 from the Hungarian National Science Foundation (OTKA).


  1. 1.
    Benedek B, Geisz N, Jäger W, Thalhammer T, Kopp B (2006) Phytomedicine 13:702–706CrossRefGoogle Scholar
  2. 2.
    Wichtl M (2002) Teedrogen 4th edition.Wiss. Verlags GesmbH, StuttgartGoogle Scholar
  3. 3.
    Rauchensteiner F, Nejati S, Werner I, Glasl S, Saukel J, Jurenitsch J, Kubelka W (2002) Sci Pharm 70:199–230Google Scholar
  4. 4.
    Seaman FC (1982) Bot Rev 48:415–416Google Scholar
  5. 5.
    Trifunović S, Aljančić I, Vajs V, Macura S, Milosavljević S (2005) Biochem Syst Ecol 33:317–322CrossRefGoogle Scholar
  6. 6.
    Trendafilova A, Todorova M, Mikhova B, Vitkova A, Duddeck H (2006) Phytochemistry 67:764–770CrossRefGoogle Scholar
  7. 7.
    Mustakerova E, Todorova M, Tsankova E (2002) Z Naturfosch 57:568–570Google Scholar
  8. 8.
    Simic N, Palic R, Vajs V, Milosavljevic S, Djokovic D (2002) J Essent Oil Res 14:76–78Google Scholar
  9. 9.
    Candan TF, Unlu M, Tepe B, Daferera D, Polissiou M, Sökmen A, Akpulat HA (2005) J Ethnopharmacol 87:215–220CrossRefGoogle Scholar
  10. 10.
    Konyalioglu S, Karamenderes CJ (2007) J Ethnopharmacol 103:221–227Google Scholar
  11. 11.
    Benedek B, Kopp B (2007) Wien Med Wochenschr 157/13–14:312–314CrossRefGoogle Scholar
  12. 12.
    Saukel J, Länger R (1992) Phyton 31:185–207Google Scholar
  13. 13.
    Saukel J, Länger R (1992) Phyton 32:47–78Google Scholar
  14. 14.
    Benedek B, Gjoncaj N, Saukel J, Kopp B (2007) Distrib Chem Biodivers 4:849–857CrossRefGoogle Scholar
  15. 15.
    Mehlführer M, Troll K, Jurenitsch J, Auer H, Kubelka W (1997) Phytochemistry 44:1067–1069CrossRefGoogle Scholar
  16. 16.
    Kubelka W, Kastner U, Glasl S, Saukel J, Jurenitsch J (1999) Biochem Syst Ecol 27:437–444CrossRefGoogle Scholar
  17. 17.
    Rothwangl K, Glasl S, Werner I, Reznicek G (2004) Sci Pharm 72:249–258Google Scholar
  18. 18.
    Glasl S, Kastner U, Jurenitsch J, Kubelka W (1999) J Chromatogr B 729:361–368CrossRefGoogle Scholar
  19. 19.
    Todorova M.N, Mikhova B, Trendafilova A, Vitkova A, Duddeck H, Anchev M (2006) Biochem Syst Ecol 34:136–143CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2008

Authors and Affiliations

  • Gergely Montsko
    • 1
  • Borbala Boros
    • 2
  • Aniko Takatsy
    • 1
  • Robert Ohmacht
    • 1
  • Sabine Glasl
    • 3
  • Liselotte Krenn
    • 3
  • Laszlo Mark
    • 1
  • Gottfried Reznicek
    • 3
  1. 1.Department of Biochemistry and Medical Chemistry, Faculty of MedicineUniversity of PecsPecsHungary
  2. 2.Institute of Pharmaceutical ChemistryUniversity of PécsPécsHungary
  3. 3.Department of Pharmacognosy, Faculty of Life SciencesUniversity of ViennaViennaAustria

Personalised recommendations