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Chromatographia

, Volume 67, Issue 3–4, pp 211–217 | Cite as

Fingerprint Analysis of Eucommia Bark by LC-DAD and LC-MS with the Aid of Chemometrics

  • Yongnian Ni
  • Yunyan Peng
  • Serge Kokot
Original

Abstract

Chromatographic fingerprints of 46 Eucommia Bark samples were obtained by liquid chromatography-diode array detector (LC-DAD). These samples were collected from eight provinces in China, with different geographical locations, and climates. Seven common LC peaks that could be used for fingerprinting this common popular traditional Chinese medicine were found, and six were identified as substituted resinols (4 compounds), geniposidic acid and chlorogenic acid by LC-MS. Principal components analysis (PCA) indicated that samples from the Sichuan, Hubei, Shanxi and Anhui—the SHSA provinces, clustered together. The other objects from the four provinces, Guizhou, Jiangxi, Gansu and Henan, were discriminated and widely scattered on the biplot in four province clusters. The SHSA provinces are geographically close together while the others are spread out. Thus, such results suggested that the composition of the Eucommia Bark samples was dependent on their geographic location and environment. In general, the basis for discrimination on the PCA biplot from the original 46 objects× 7 variables data matrix was the same as that for the SHSA subset (36 × 7 matrix). The seven marker compound loading vectors grouped into three sets: (1) three closely correlating substituted resinol compounds and chlorogenic acid; (2) the fourth resinol compound identified by the OCH3 substituent in the R4 position, and an unknown compound; and (3) the geniposidic acid, which was independent of the set 1 variables, and which negatively correlated with the set 2 ones above. These observations from the PCA biplot were supported by hierarchical cluster analysis, and indicated that Eucommia Bark preparations may be successfully compared with the use of the HPLC responses from the seven marker compounds and chemometric methods such as PCA and the complementary hierarchical cluster analysis (HCA).

Keywords

Column liquid chromatography LC-DAD LC-MS Fingerprinting Principal component analysis Eucommia Bark 

Notes

Acknowledgments

The authors gratefully acknowledge the financial support of this study by the National Natural Science Foundation of China (NSFC20365002 and NSFC20562009), the State Key Laboratories of Electroanalytical Chemistry of Changchun Applied Chemical Institute (SKLEAC2004-3) and the Chemo/Biosensing and Chemometrics of Hunan University (SKLCBC2005-22), the Jiangxi Provincial Natural Science Foundation (JXNSF062004), and the program for Changjiang Scholars and Innovative Research Team in Universities (IRT0540).

References

  1. 1.
    Wen KC, Huang CY, Lu FL (1993) J Chromatogr A 631:241–250CrossRefGoogle Scholar
  2. 2.
    Drasar P, Moravcova J (2004) J Chromatogr B 812:3–21Google Scholar
  3. 3.
    Normile D (2004) Am J Med 116:478–485CrossRefGoogle Scholar
  4. 4.
    Stephen B, Richard K (2004) Am J Med 116:478–485CrossRefGoogle Scholar
  5. 5.
    World Health Organization (1991) Guidelines for the Assessment of Herbal Medicines, Munich, 28.6.1991. WHO, GenevaGoogle Scholar
  6. 6.
    Cheng YY, Chen MJ, Tong WD (2003) J Chem Inf Comput Sci 43:1068–1076CrossRefGoogle Scholar
  7. 7.
    Schoonjans V, Taylor N, Hudson BD, Massart DL (2002) J Pharmaceut Biomed Anal 28:537–548CrossRefGoogle Scholar
  8. 8.
    Donohue MJ, Smallwood AW, Pfaller S, Rodgers M, Shoemaker JA, (2006) J Microbiol Meth 65:380–389CrossRefGoogle Scholar
  9. 9.
    Liang YZ, Xie PS, Chan K (2007) J Chromatogr B 812:53–70Google Scholar
  10. 10.
    Ni YN, Zhang LS, Churchill J, Kokot S (2007) Talanta 72:1533–1539CrossRefGoogle Scholar
  11. 11.
    Cheng YY, Chen MJ, Tong WD (2003) J Chem Inf Comput Sci 43:1959–1965CrossRefGoogle Scholar
  12. 12.
    Shen N (2005) Shen Nong Ben Cao Jing, Gu GG (ed), Lanzhou University Press, LanzhouGoogle Scholar
  13. 13.
    Li SZ (2005) Ben Cao Gang Mu: a translation version in modern Chinese (CD-ROM edition). Inner Mongolia Press, HolhotGoogle Scholar
  14. 14.
    Chinese Pharmacopoeia Committee (2005) Chinese Pharmacopoeia. Chemical Industry Press, BeijingGoogle Scholar
  15. 15.
    Kwan CY, Chen CX, Deyama T (2004) Vasc Pharmacol 11:229–235Google Scholar
  16. 16.
    Kwan CY, Zhang WB, Deyama T, Nishibe S (2004) Naunyn-Schmied Arch Pharmacol 369:206–211CrossRefGoogle Scholar
  17. 17.
    Li YM, Metrori K (1999) Bio Pharm Bull 22:582–585Google Scholar
  18. 18.
    Yang J, Kato K, Noguchi K, Dairaku N, Koike T, Iijima K, Imatani A, Sekine H, Ohara S, Sasano H, Shimosegawa T (2003) Life Sci 73:3245–3256CrossRefGoogle Scholar
  19. 19.
    Hsieh CL, Yen GC (2000) Life Sci 66:1387–1400CrossRefGoogle Scholar
  20. 20.
    Hajimahmoodi M, Heyden YV, Sadeghi N, Jannat B, Oveisi MR, Shahbazian S (2005) Talanta 66:1108–1116CrossRefGoogle Scholar
  21. 21.
    Gu M, Su ZG, Fan OY (2006) J Liq Chromatogr Related Technol 29:1503–1514CrossRefGoogle Scholar
  22. 22.
    Zhou X, Peng JY, Fan GR, Wu YT (2005) J Chromatogr A 1092:216–221CrossRefGoogle Scholar
  23. 23.
    Gu M, Zhang SF, Su ZG, Chen Y, Fan OY (2004) J Chromatogr A 1057:133–140CrossRefGoogle Scholar
  24. 24.
    Gong F, Liang YZ, Xie PS, Chau FT (2004) J Chromatogr A 1029:173–183CrossRefGoogle Scholar
  25. 25.
    Cheng YY, Chen MJ, Wu YJ (2002) Acta Chim Sin 60:2017–2021Google Scholar
  26. 26.
    Marbach R, Heise M (1990) Chemom Intell Lab Sys 9:45–63CrossRefGoogle Scholar
  27. 27.
    Martens H, Naes T (1991) Multivariate Calibration. John Wiley and Sons, New YorkGoogle Scholar
  28. 28.
    Feng Y, Zhang L, Cai JN, Wang ZT, Xu L, Zhang ZX (2001) Talanta 53:1155–1162CrossRefGoogle Scholar
  29. 29.
    Nie L, Luo GA, Cao J, Wang YM (2004) Acta Pharm Sin 39:136–139Google Scholar
  30. 30.
    Brereton RG (2003) Chemometrics: Data analysis for the laboratory and chemical plant. John Wiley and Sons, New York, pp 184Google Scholar
  31. 31.
    Gan F, Ye RY (2006) J Chromatog A 1104:100–105CrossRefGoogle Scholar
  32. 32.
    Deyama T (1983) Chem Pharm Bull 31:2993–2997Google Scholar
  33. 33.
    Deyama T, Ikawa T, Nishibe S (1985) Chem Pharm Bull 33:3651–3657Google Scholar
  34. 34.
    Deyama T, Ikawa T, Kitagawa S, Nishibe S (1986) Chem Pharm Bull 34:523–527Google Scholar
  35. 35.
    Deyama T, Ikawa T, Kitagawa S, Nishibe S (1986) Chem Pharm Bull 34:4933–4938Google Scholar
  36. 36.
    Geladi P, Kowalski BR (1986) Anal Chim Acta 185:1–17CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2007

Authors and Affiliations

  1. 1.Department of ChemistryNanchang UniversityNanchangPeople’s Republic of China
  2. 2.Inorganic Materials Program, School of Physical and Chemical SciencesQueensland University of TechnologyBrisbaneAustralia

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