, Volume 67, Issue 3–4, pp 211–217 | Cite as

Fingerprint Analysis of Eucommia Bark by LC-DAD and LC-MS with the Aid of Chemometrics

  • Yongnian Ni
  • Yunyan Peng
  • Serge Kokot


Chromatographic fingerprints of 46 Eucommia Bark samples were obtained by liquid chromatography-diode array detector (LC-DAD). These samples were collected from eight provinces in China, with different geographical locations, and climates. Seven common LC peaks that could be used for fingerprinting this common popular traditional Chinese medicine were found, and six were identified as substituted resinols (4 compounds), geniposidic acid and chlorogenic acid by LC-MS. Principal components analysis (PCA) indicated that samples from the Sichuan, Hubei, Shanxi and Anhui—the SHSA provinces, clustered together. The other objects from the four provinces, Guizhou, Jiangxi, Gansu and Henan, were discriminated and widely scattered on the biplot in four province clusters. The SHSA provinces are geographically close together while the others are spread out. Thus, such results suggested that the composition of the Eucommia Bark samples was dependent on their geographic location and environment. In general, the basis for discrimination on the PCA biplot from the original 46 objects× 7 variables data matrix was the same as that for the SHSA subset (36 × 7 matrix). The seven marker compound loading vectors grouped into three sets: (1) three closely correlating substituted resinol compounds and chlorogenic acid; (2) the fourth resinol compound identified by the OCH3 substituent in the R4 position, and an unknown compound; and (3) the geniposidic acid, which was independent of the set 1 variables, and which negatively correlated with the set 2 ones above. These observations from the PCA biplot were supported by hierarchical cluster analysis, and indicated that Eucommia Bark preparations may be successfully compared with the use of the HPLC responses from the seven marker compounds and chemometric methods such as PCA and the complementary hierarchical cluster analysis (HCA).


Column liquid chromatography LC-DAD LC-MS Fingerprinting Principal component analysis Eucommia Bark 



The authors gratefully acknowledge the financial support of this study by the National Natural Science Foundation of China (NSFC20365002 and NSFC20562009), the State Key Laboratories of Electroanalytical Chemistry of Changchun Applied Chemical Institute (SKLEAC2004-3) and the Chemo/Biosensing and Chemometrics of Hunan University (SKLCBC2005-22), the Jiangxi Provincial Natural Science Foundation (JXNSF062004), and the program for Changjiang Scholars and Innovative Research Team in Universities (IRT0540).


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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2007

Authors and Affiliations

  1. 1.Department of ChemistryNanchang UniversityNanchangPeople’s Republic of China
  2. 2.Inorganic Materials Program, School of Physical and Chemical SciencesQueensland University of TechnologyBrisbaneAustralia

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