, Volume 67, Supplement 1, pp 63–68 | Cite as

ITP Determination of Potassium and Ammonium in Fertilizers and Silage

  • Michaela Nováková
  • Ludmila Křivánková
  • Irena Skalická
  • Martin Bartoš
  • Karel Vytřas


A method for isotachophoretic determination of potassium and ammonium cations in fertilizers and silage was developed. A capillary of 0.4 mm i.d. and 100 mm effective length made of fluorinated ethylene–propylene copolymer was filled with an electrolyte system consisting of 10 mmol L−1 RbOH + 0.1% (w/v) hydroxyethylcellulose, adjusted to pH 9.0 with l-histidine (leading electrolyte) and 10 mmol L−1 lithium citrate (terminating electrolyte). Using contactless conductivity detection, the calibration curves in the tested concentration range up to 0.5 mmol L−1 were linear for both cations. The concentration detection limits for potassium and ammonium were 2.9 and 2.7 μmol L−1, respectively. RSD values of step lengths (n = 6) were 1.3% for potassium and 1.5% for ammonium. The separation time was about 20 min. Similar results were obtained with cesium cation used as the leading ion, however, in the system with rubidium better resolution of other cations present in tested matrices was reached. The elaborated method is simple to perform, sufficiently sensitive and accurate and can be recommended as an alternative procedure to the methods used so far for the determination of potassium and ammonium.


Capillary electrophoresis Isotachophoresis Ammonium Potassium 



This work was supported by grant No. 203/05/2106 of the Czech Science Foundation, grant IAA 400310703 of the Academy of Science of the Czech Republic, the Institutional Research Plan Z40310501, and the grant of the Czech Ministry of Education, Youths and Sports (project. No. MSM0021627502).


  1. 1.
    Remy H (1970) Handbook of treatise on inorganic chemistry. ALGC, BrusselsGoogle Scholar
  2. 2.
    Cotton FA, Wilkinson G (1988) Handbook of inorganic chemistry. Wiley, New YorkGoogle Scholar
  3. 3.
    Stover FS (1984) J Chromatogr A298:203–210CrossRefGoogle Scholar
  4. 4.
    Valášková I, Havránek E (1999) J Chromatogr A 836:201–208CrossRefGoogle Scholar
  5. 5.
    Kalavská D, Holoubek I (1989) Analýza vôd (water analysis). Acta, BratislavaGoogle Scholar
  6. 6.
    Malát M (1973) Absorpční anorganická fotometrie. Academia, PrahaGoogle Scholar
  7. 7.
    Amin M, Lim LW, Takeuchi T (2007) Talanta 71:1470–1475CrossRefGoogle Scholar
  8. 8.
    Svensson S, Isacsson ACh, Ljunqkvist G, Torén K, Olin AC (2005) J Chromatogr B 814:173–177CrossRefGoogle Scholar
  9. 9.
    Hirokawa T, Okamoto H, Gosyo Y, Tsuda T, Timerbaev AR (2007) Anal Chim Acta 581:83–88CrossRefGoogle Scholar
  10. 10.
    Fung YS, Lau KM (2006) J Chromatogr A 1118:144–150CrossRefGoogle Scholar
  11. 11.
    Javorský P (1976) Chemické rozbory v zemědělských laboratořích. MZVž ČSR, PrahaGoogle Scholar
  12. 12.
    Kaniansky D, Zelenský I, Valášková I, Marák J, Zelenská V (1990) J Chromatogr A 502:143–153CrossRefGoogle Scholar
  13. 13.
    Fukushi K, Hiiro K (1990) J Chromatogr A 523:281–292CrossRefGoogle Scholar
  14. 14.
    Matejovič I, Polonský J (1988) J Chromatogr A 438:454–458CrossRefGoogle Scholar
  15. 15.
    Lemmens AAG, Everaerts FM, Venema JW, Jonker HD (1988) J Chromatogr A 439:423–429CrossRefGoogle Scholar
  16. 16.
    Zelenský I, Pešlová H, Kaniansky D (1989) Agrochémia 29:160–164Google Scholar
  17. 17.
    Fukushi K, Hiiro K (1988) Talanta 35:55–56CrossRefGoogle Scholar
  18. 18.
    Blatný P, Kvasnička F, Loučka R, Šafářová H (1997) J Agric Food Chem 45:3554–3558CrossRefGoogle Scholar
  19. 19.
    Fukushi K, Jikro K (1988) Fresenius Z Anal Chem 332:125–129CrossRefGoogle Scholar
  20. 20.
    Hirokawa T, Nishino M, Aoki N, Kiso Y, Sawamoto Y, Yagi T, Akiyama J (1983) J Chromatogr A 271:D1–D100CrossRefGoogle Scholar
  21. 21.
    Beckers JL, Everaerts FM (1972) J Chromatogr A 68:207–230CrossRefGoogle Scholar
  22. 22.
    Kotrlý S, Šůcha L (1985) Handbook of chemical equilibria in analytical chemistry. Ellis Horwood, ChichesterGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2008

Authors and Affiliations

  • Michaela Nováková
    • 1
    • 2
  • Ludmila Křivánková
    • 1
  • Irena Skalická
    • 2
  • Martin Bartoš
    • 2
  • Karel Vytřas
    • 2
  1. 1.Institute of Analytical ChemistryAcademy of Sciences of the Czech RepublicBrnoCzech Republic
  2. 2.Department of Analytical Chemistry, Faculty of Chemical TechnologyUniversity of PardubicePardubiceCzech Republic

Personalised recommendations