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Chromatographia

, Volume 67, Issue 3–4, pp 231–235 | Cite as

LC-MS-MS Determination of Cyclo{(2S)-2-amino-8-[(aminocarbonyl)hydrazono] decanoyl-1-l-tryptophyl-l-isoleucyl-(2R)-2-piperidinecarbonyl} a Novel Histone Deacetylase Inhibitor in Rat Serum

  • Beom Soo Shin
  • Deok Ki Hong
  • Jong Hwan Kwak
  • John Kim
  • Sun Dong Yoo
Original

Abstract

A rapid and sensitive liquid chromatography-tandem mass spectrometry assay was developed for the determination of a novel histone deacetylase inhibitor, cyclo{(2S)-2-amino-8-[(aminocarbonyl)hydrazono]decanoyl-1-l-tryptophyl-l-isoleucyl-(2R)-2-piperidinecarbonyl} (SD-2007), in rat serum. The mobile phase consisted of acetonitrile and ammonium formate (10 mM) (85:15 v/v), and the flow rate was 0.25 mL min−1. Chromatographic separations were achieved by isocratic elution on a C18 column. Multiple reaction monitoring was based on the transition of m/z = 681.8 → 83.6 for SD-2007 and 372.1 → 176.1 for trazodone (internal standard). A linearity was observed over a concentration range from 2 to 1,000 ng mL−1 (r 2 > 0.999), with the lower limit of quantification at 2 ng mL−1 with 100 μL of rat serum. The mean intra- and inter-day assay accuracy ranged from 98.5–109.7% to 95.2–102.7%, respectively, and the mean intra- and inter-day precision was between 4.3–11.3% and 2.9–13.3%. The developed assay was applied to a pharmacokinetic study of SD-2007 in rats after intravenous injection (dose 4 mg kg−1).

Keywords

Column liquid chromatography-mass spectrometry LC-MS-MS Pharmacokinetics Histone deacetylase inhibitor SD-2007 

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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2007

Authors and Affiliations

  • Beom Soo Shin
    • 1
  • Deok Ki Hong
    • 2
  • Jong Hwan Kwak
    • 2
  • John Kim
    • 3
  • Sun Dong Yoo
    • 2
  1. 1.College of PharmacyCatholic University of DaeguGyeongbukSouth Korea
  2. 2.College of PharmacySungkyunkwan UniversitySuwon, Kyeonggi-doSouth Korea
  3. 3.Clinical ResearchHanmi Pharm. Co. LtdSeoulSouth Korea

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