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Chromatographia

, Volume 67, Issue 1–2, pp 143–146 | Cite as

RP-LC Determination of Atomoxetine HCl from its Pharmaceutical Dosage Form

  • Sudhir S. Kamat
  • Vishal B. Choudhari
  • Vinayak T. Vele
  • Swarup S. Prabhune
Full Short Communication

Abstract

A rapid, simple and sensitive high-performance liquid chromatographic method (HPLC) has been developed to assay atomoxetine HCl in capsules. The HPLC analysis used a reversed phase C18 (150 × 4.6 mm i.d. 5 μm particle size) analytical column and a mobile phase consisting of monobasic potassium dihydrogen orthophosphate and acetonitrile (95:5 v/v), with UV detection at 269 nm. The validation data showed that the assay is sensitive, specific and reproducible for determination of atomoxetine HCl in this dosage form. Calibration curves were linear from 1 to 10 μg mL−1 (R 2 > 0.997). The accuracy of the method ranged from 98.13 to 101.5%. Mean inter- and intra-assay relative standard deviations (RSD) were less than 1.0%. The proposed method provided an accurate and precise analysis of atomoxetine HCl in its pharmaceutical dosage form.

Keywords

Column liquid chromatography Pharmaceutical formulation Atomoxetine HCl 

Notes

Acknowledgment

The authors are thankful to Dr. Arvind A. Natu (Sr. Scientist, NCL, Pune), Dr. Lalit Anande, Dr. Ashok Mehta and Prof. Dr. R.T. Sane for their valuable guidance and cooperation.

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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2007

Authors and Affiliations

  • Sudhir S. Kamat
    • 1
  • Vishal B. Choudhari
    • 1
  • Vinayak T. Vele
    • 1
  • Swarup S. Prabhune
    • 1
  1. 1.Therapeutic Drug Monitoring LaboratoryMumbaiIndia

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