Chromatographia

, Volume 66, Issue 9–10, pp 669–676 | Cite as

Simultaneous Determination of Trichothecenes A, B, and D in Maize Food Products by LC–MS–MS

  • Alessandra Gentili
  • Fulvia Caretti
  • Giuseppe D’Ascenzo
  • Lucia Mainero Rocca
  • Stefano Marchese
  • Stefano Materazzi
  • Daniela Perret
Original

Abstract

Two new methods of analysis, based on liquid chromatography–tandem mass spectrometry, for simultaneous determination of trichothecenes A, B, and D in maize flour and oil have been developed and validated in accordance with European Commission decision 2002/657/EC (recovery, CCα, CCβ, and precision). The trichothecenes were extracted from maize flour by matrix solid-phase dispersion, with recoveries ≥79%, and from maize oil by liquid–liquid extraction, with recoveries ≥78%. Limits of quantitation ranged between 0.03 and 50 μg kg−1, depending on the electrospray response to each analyte and on the matrix. Monitoring of flour and oil samples with this HPLC–MS–MS method revealed the presence of deoxynivalenol, T-2 toxin, and diacetoxyscirpenol at very low concentrations.

Keywords

Column liquid chromatography Electrospray tandem mass spectrometry Matrix solid-phase dispersion Trichothecenes A, B, and D Maize flour and oil 

Notes

Acknowledgments

This work was supported by the Ministero Italiano Delle Politiche Agricole e Forestali (MIPAF), “Qualità Alimentare” project.

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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2007

Authors and Affiliations

  • Alessandra Gentili
    • 1
  • Fulvia Caretti
    • 1
  • Giuseppe D’Ascenzo
    • 1
  • Lucia Mainero Rocca
    • 1
  • Stefano Marchese
    • 1
  • Stefano Materazzi
    • 1
  • Daniela Perret
    • 1
  1. 1.Dipartimento di ChimicaUniversità “La Sapienza” di RomaRomeItaly

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