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Simultaneous LC–MS–MS Determination of Zolmitriptan and Its Active Metabolite N-Desmethylzolmitriptan in Human Plasma

Abstract

A specific and sensitive liquid chromatography-electrospray ionization tandem mass spectrometry method was developed for the determination of zolmitriptan and N-desmethylzolmitriptan in human plasma. The analytes and the internal standard (IS) paroxetine were extracted by liquid–liquid extraction with a mixture of saturated ethyl acetate:dichloromethane (4:1) and were separated using an isocratic mobile phase on a XTerra RP18 column. The mobile phase used was acetonitrile: 5 mM ammonium acetate: formic acid (50:50:0.053, v/v/v). Zolmitriptan and N-desmethylzolmitriptan in a range of 0.25–20 ng mL−1 were easily quantified. The validated method can be applied to pharmacokinetic and bioequivalence studies.

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References

  1. 1.

    Rapoport AM, Cady RK, Mathew NT (1995) Cephalalgia 15:221–226

    Google Scholar 

  2. 2.

    Seaber E, On N, Dixon RM (1997) Br J Clin Pharmacol 43:579–587

    Article  CAS  Google Scholar 

  3. 3.

    Chen J, Jiang XG, Jiang WM, Mei N, Gao XL, Zhang QZ (2004) J Pharm Biomed Anal 35:639–645

    Article  CAS  Google Scholar 

  4. 4.

    Clement EM, Franklin M (2002) J Chromatogr B 766:339–343

    Article  CAS  Google Scholar 

  5. 5.

    Vishwanathan K, Barlett MG, Stewart JT (2000) Rapid Commun Mass Spectrom 14:168–172

    Article  CAS  Google Scholar 

  6. 6.

    Zhang Z, Xu F, Tian Y, Li W, Mao G (2004) J Chromatogr B 813:227–233

    Article  CAS  Google Scholar 

  7. 7.

    Chen X, Liu D, Luan Y, Jin F, Zhong D (2006) J Chromatogr B 832:30–35

    Article  CAS  Google Scholar 

  8. 8.

    Karnes HT, Shiu G, Shah VP (1991) Pharm Res 8:421–426

    Article  CAS  Google Scholar 

Download references

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Correspondence to B. Kılıç.

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Application of Separation Techniques in Turkey

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Kılıç, B., Özden, T., Toptan, S. et al. Simultaneous LC–MS–MS Determination of Zolmitriptan and Its Active Metabolite N-Desmethylzolmitriptan in Human Plasma. Chroma 66, 129–133 (2007). https://doi.org/10.1365/s10337-007-0293-8

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Keywords

  • Column liquid chromatography–mass spectrometry
  • LC–MS–MS
  • Pharmacokinetics
  • Human plasma
  • Zolmitriptan
  • N-Desmethylzolmitriptan