Chromatographia

, Volume 66, Issue 3–4, pp 231–235 | Cite as

Kinetics of Adsorption of Thymopentin on a Gel-Type Strong Cation-Exchange Resin

  • Xin Li
  • Lei Zhang
  • Yonghui Chang
  • Shubao Shen
  • Hanjie Ying
  • Pingkai Ouyang
Original

Abstract

The ion-exchange kinetics have been determined for adsorption of thymopentin on a gel-type sulfonated styrene–divinylbenzene resin converted to the ammonium form. Batch equilibrium and kinetic experiments were performed in chloride ion solutions of different concentration. Equilibrium data revealed isotherms were a good fit to the constant separation factor isotherm. Because of the high capacity and low cost of the resin its use for uptake of thymopentin was economically feasible. Kinetic data were compared with the predictions from the Nernst–Planck and Fick models. The intraparticle and effective diffusivity of thymopentin were obtained from these models.

Keywords

Ion exchange Gel-type resin Cation exchange Thymopentin Kinetics 

Abbreviations

C

Concentration of peptide in the liquid phase (mmol L−1)

CT

Maximum peptide equilibrium concentration in the liquid phase (mmol L−1)

De

Effective diffusivity (cm2 s−1)

D0

Solution diffusivity (cm2 s−1)

F

Faraday constant

K

Separation factor constant

N

Flux of ion (mmol cm−2 s−1)

Qtotal

Total ion-exchange capacity (mmol g−1 wet resin)

q

Concentration of ion in the resin (mmol g−1 wet resin)

q*

Equilibrium concentration of ion in the resin (mmol g−1 wet resin)

qt

Maximum peptide equilibrium concentration in the resin (mmol g−1 wet resin)

r

Special coordinate in the resin (mm)

R

Dimensionless special coordinate in the resin

Rp

Resin radius (mm)

\( {\Re } \)

Ideal gas constant

X

Dimensionless peptide concentration in liquid phase

Y

Dimensionless peptide concentration in the resin

y

Dimensionless concentration of ion in the resin

y*

Dimensionless equilibrium concentration of ion in the resin

z

Charge on the ion

Greek Letters

δ

Ratio of the diffusivities of ions j and i

τ

Dimensionless time

φ

Electric potential

Notes

Acknowledgments

This work was supported by the National High Technology Research and Development Program of China (No. 2003AA219042) and the Doctor Fund of Nanjing University of Technology (No. BSCX200607).

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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2007

Authors and Affiliations

  • Xin Li
    • 1
  • Lei Zhang
    • 1
  • Yonghui Chang
    • 1
  • Shubao Shen
    • 1
  • Hanjie Ying
    • 1
  • Pingkai Ouyang
    • 1
  1. 1.College of Life Science and Pharmaceutical EngineeringNanjing University of TechnologyNanjingChina

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