Chromatographia

, Volume 66, Supplement 1, pp 153–157 | Cite as

HPLC Quantification of Vitexine-2″-O-rhamnoside and Hyperoside in Three Crataegus Species and Their Antimicrobial and Antiviral Activities

  • I. Orhan
  • B. Özçelik
  • M. Kartal
  • B. Özdeveci
  • H. Duman
Full Short Communication

Abstract

Crataegus sp., also known as “hawthorn”, is one of the most prescribed herbal drugs and approved to be used against congestive heart failure. The extract is required to be standardized according to its flavonoid (vitexine-2″-O-rhamnoside and hyperoside) and oligomeric procyanidin content by the European Pharmacopoeia (EP). In this study, the leaves and berries of three Crataegus species including C. aronia var. aronia, C. monogyna ssp. monogyna (from two different localities) as well as C. pseudoheterophylla growing in Turkey were evaluated for their vitexine-2″-O-rhamnoside and hyperoside content by reversed-phase HPLC as well as their total procyanidin amount by a spectrophotometric method. In addition, antibacterial, antifungal, and antiviral activities of the extracts were also determined. Our results indicated that C. monogyna ssp. monogyna samples of both localities met the criteria required by the EP for their total flavonoid amount (2.49 and 2.86%) and total proanthocyanidin content (1.56 and 2.22%). According to data we obtained, the extracts have been verified to be highly effective against Candida albicans and Herpes simplex virus.

Keywords

Column liquid chromatography Antimicrobial activity Hyperoside Crataegus Vitexine-2″-O-rhamnoside 

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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2007

Authors and Affiliations

  • I. Orhan
    • 1
  • B. Özçelik
    • 2
  • M. Kartal
    • 3
  • B. Özdeveci
    • 1
  • H. Duman
    • 4
  1. 1.Department of Pharmacognosy, Faculty of PharmacyGazi UniversityAnkaraTurkey
  2. 2.Department of Pharmaceutical Microbiology, Faculty of PharmacyGazi UniversityAnkaraTurkey
  3. 3.Department of Pharmacognosy, Faculty of PharmacyAnkara UniversityAnkaraTurkey
  4. 4.Department of Biology, Faculty of Science and ArtGazi UniversityAnkaraTurkey

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