, Volume 66, Supplement 1, pp 37–43 | Cite as

A Validated HPLC Method with Fluorescence Detection for the Determination of Droperidol in Pharmaceutical Tablets, Human Serum, and Human Milk

  • D. Yeniceli
  • D. Dogrukol-Ak
  • M. Tuncel


A sensitive and simple HPLC method with fluorimetric detection has been developed for determination of droperidol in pharmaceutical tablets, human serum, and human milk. Chromatography was performed on a 100 mm × 3 mm i.d. C18 column with methanol–water, 30:70 (v/v), pH 3.5, as mobile phase at a flow-rate of 0.8 mL min−1. The injection volume was 5 μL and detection was by monitoring emission at 324 nm after excitation at 283 nm. Droperidol and p-hydroxybenzoic acid (internal standard) eluted after 5.3 and 6.1 min, respectively. The method was validated over the concentration range 1.14 × 10−7 to 9.12 × 10−6 M. Selectivity was good and the limits of detection and quantitation of the method were approximately 3.54 × 10−8 and 1.07 × 10−7 M, respectively, corresponding to 13 and 40 ng mL−1. The applicability of the method to determination of droperidol in pharmaceuticals, human serum, and human milk was demonstrated.


Column liquid chromatography Fluorimetric detection Pharmaceuticals in human serum and human milk Droperidol 



The authors are grateful to the Research Council of Anadolu University for their support of our project.


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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2007

Authors and Affiliations

  1. 1.Faculty of Pharmacy, Department of Analytical ChemistryAnadolu UniversityEskisehirTurkey

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