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Chromatographia

, Volume 66, Supplement 1, pp 37–43 | Cite as

A Validated HPLC Method with Fluorescence Detection for the Determination of Droperidol in Pharmaceutical Tablets, Human Serum, and Human Milk

  • D. Yeniceli
  • D. Dogrukol-Ak
  • M. Tuncel
Original

Abstract

A sensitive and simple HPLC method with fluorimetric detection has been developed for determination of droperidol in pharmaceutical tablets, human serum, and human milk. Chromatography was performed on a 100 mm × 3 mm i.d. C18 column with methanol–water, 30:70 (v/v), pH 3.5, as mobile phase at a flow-rate of 0.8 mL min−1. The injection volume was 5 μL and detection was by monitoring emission at 324 nm after excitation at 283 nm. Droperidol and p-hydroxybenzoic acid (internal standard) eluted after 5.3 and 6.1 min, respectively. The method was validated over the concentration range 1.14 × 10−7 to 9.12 × 10−6 M. Selectivity was good and the limits of detection and quantitation of the method were approximately 3.54 × 10−8 and 1.07 × 10−7 M, respectively, corresponding to 13 and 40 ng mL−1. The applicability of the method to determination of droperidol in pharmaceuticals, human serum, and human milk was demonstrated.

Keywords

Column liquid chromatography Fluorimetric detection Pharmaceuticals in human serum and human milk Droperidol 

Notes

Acknowledgments

The authors are grateful to the Research Council of Anadolu University for their support of our project.

References

  1. 1.
    Weaver CS, Jones JB, Chisholm CD, Foley MJ, Giles BK, Somerville GG, Brizendine EJ, Cordell WH (2004) J Emerg Med 26:145–150CrossRefGoogle Scholar
  2. 2.
    Flood P, Coates KM (2002) Anesthesiology 96:987–993CrossRefGoogle Scholar
  3. 3.
    Trabelsi H, Raouafi F, Limam M, Bouzouita K (2002) J Pharm Biomed Anal 29:239–245CrossRefGoogle Scholar
  4. 4.
    Haemers A, Van den Bossche WJ (1969) J Pharm Pharmacol 21:531–532Google Scholar
  5. 5.
    Baeyens W (1977) Analyst 102:525–530CrossRefGoogle Scholar
  6. 6.
    Pap S, Kauffmann JM, Vire JC, Patriarche GJ, Prete MP (1984) J Pharm Belg 39:335–340Google Scholar
  7. 7.
    Hopkala H, Przyborowski L (1989) Pharmazie 44:65Google Scholar
  8. 8.
    Biryol I, Ozkan SA (1997) J Pharm Biomed Anal 15:1695–1701CrossRefGoogle Scholar
  9. 9.
    Rosenfeld J, Kawai M, Rigg JRA, Khandelwal JK (1976) J Chromatogr 129:387–392CrossRefGoogle Scholar
  10. 10.
    Heusler H (1985) J Chromatogr 340:273–319CrossRefGoogle Scholar
  11. 11.
    Quaglio MP, Bellini AM, Lambardi F (1982) Boll Chim Farm 121:276–284Google Scholar
  12. 12.
    Kurzawa M, Kowalczyk-Marzec A, Szlyk M (2004) Chem Anal 49:91–99Google Scholar
  13. 13.
    Dolezalova M (1984) J Chromatogr 286:323–330CrossRefGoogle Scholar
  14. 14.
    Wilhelm D, Kemper A (1990) J Chromatogr 525:218–224CrossRefGoogle Scholar
  15. 15.
    Tan ST, Boniface PJ (1990) J Chromatogr 532:181–186CrossRefGoogle Scholar
  16. 16.
    Guichard J, Pantex G, Dubost J, Baltassat P, Roche C (1993) J Chromatogr 612:269–275CrossRefGoogle Scholar
  17. 17.
    Kumar K, Ballantyne JA, Baker AB (1996) J Pharm Biomed Anal 14:1529–1533CrossRefGoogle Scholar
  18. 18.
    Trabelsi H, Guettat S, Bouzouita K, Safta F (2002) J Pharm Biomed Anal 28:453–462CrossRefGoogle Scholar
  19. 19.
    Lee DKT, Chiou AHJ, Wang DP (2005) J Food Drug Anal 13:93–95Google Scholar
  20. 20.
    Higashi Y, Kitahara M, Fujii Y (2006) Biomed Chromatogr 20:166–172CrossRefGoogle Scholar
  21. 21.
    Tuncel M, Dogrukol-Ak D, Yeniceli D, Uslu B, Dogan B (2006) Chromatographia 63:507–511CrossRefGoogle Scholar
  22. 22.
    Rossi DT, Wright DS (1997) J Pharm Biomed Anal 15:495–504CrossRefGoogle Scholar
  23. 23.
    ICH Topic Q2A, Validation of analytical procedures: methodology. CPMP/ICH/281/95Google Scholar
  24. 24.
    Baeyens W, De Moerloose P (1979) Anal Chim Acta 110:261–270CrossRefGoogle Scholar
  25. 25.
    US Pharmacopeial Convention (2000) US Pharmacopeia, 24th edn. US Pharmacopeial Convention, Rockville, 2001–2002Google Scholar
  26. 26.
    Yeniceli D, Dogrukol-Ak D, Tuncel M (2006) J Pharm Biomed Anal 40:197–201CrossRefGoogle Scholar
  27. 27.
    Shah VP, Midha KK, Dighe S, McGilveray IJ, Skelly JP, Jacobi A (1992) J Pharm Sci 81:309–312CrossRefGoogle Scholar
  28. 28.
    Grunwald Z, Torjman M, Schieren H, Bartkowski RR (1993) Anesth Analg 76:1238–1242CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2007

Authors and Affiliations

  1. 1.Faculty of Pharmacy, Department of Analytical ChemistryAnadolu UniversityEskisehirTurkey

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