, Volume 66, Supplement 1, pp 45–49 | Cite as

Isocratic Reverse-Phase HPLC for Determination of Organic Acids in Kargı Tulum Cheese

  • N. Dinkci
  • A. S. Akalın
  • S. Gönc
  • G. Ünal


Reverse-phase HPLC was assessed for the determination of most important organic acids in Kargı Tulum cheese. The method with a simple treatment of the sample succeeded in eluting nine organic acids in a single chromatographic run with the aid of buffer–acetonitrile gradient. A gradient programme using a mobile phase of aqueous 0.5% (w/v) (NH4)2HPO4–0.4% (v/v) acetonitrile at pH 2.24 was used to separate organic acids on a C18 column. The best flow rate–time program was applied to take the chromatogram in 35 min with a high linearity and excellent repeatability. The main organic acids of Kargı Tulum cheese were lactic, acetic, citric, propionic and formic acids. Pyruvic, uric and orotic acids were found to be lower in content while butyric acid was not detected in the samples.


Column liquid chromatography Organic acids Cheese 



The authors wish to thank the Mayor of Kargı, A. Hamdi Akpınar and the Agriculture Engineer Sevtap Ökten for their assistance on the cheese production and obtaining cheese samples.


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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2007

Authors and Affiliations

  1. 1.Department of Dairy Technology, Faculty of AgricultureEge UniversityIzmirTurkey

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