Development of a Selective LC Method for the Determination of Pravastatin Sodium
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A novel, simple and rapid stability-indicating high-performance liquid chromatographic (HPLC) method for pravastatin sodium (PRA) was successfully developed and validated for the assay of in tablets. Chromatographic separation was achieved isocratically on a C18 column (150 mm × 4.6 mm) utilizing a mobile phase of methanol-phosphate buffer (pH 7; 0.02 M) (57:43, v/v) at a flow rate of 1.0 mL min−1 with UV detection at 238 nm. A linear response (r = 0.9999) was observed in the range of 1–5 µg mL−1. The method showed good recoveries (100.50%) and the relative standard deviation of intra and inter-day were 1.40%. The method can be used for both quality control assay of pravastatin in tablets and for stability studies as the method separates pravastatin from its degradation products and tablet excipients.
KeywordsColumn liquid chromatography Validation Quality control Pravastatin
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