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Chromatographia

, Volume 63, Issue 11–12, pp 605–608 | Cite as

Determination of Gatifloxacin in Semen by HPLC with Diode-Array and Fluorescence Detection

  • M. L. Salazar Cavazos
  • L. Y. Colunga González
  • G. Gallegos de Lerma
  • N. Waksman de Torres
Article

Abstract

The objective of this work was to develop an analytical HPLC method, using DAD and fluorescence detection, for determination of gatifloxacin in semen. A reversed-phase column was used with 90:10 water-acetonitrile, containing 10 mM TBA and 25 mM citric acid, as mobile phase. Semen was deproteinized with acetonitrile. Recovery was 95 ± 10%. The limits of quantification by DAD and fluorescence were 2.3 and 0.03 μg.mL−1 respectively, with RSD of 3.4% for DAD and 2.8% for fluorescence. The method with fluorescence detection was used for quantification of gatifloxacin in the semen of patients under treatment.

Keywords

Column liquid chromatography Diode-array and fluorescence detection Fluoroquinolones Gatifloxacin in semen 

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Copyright information

© Friedr. Vieweg&Sohn/GWV Fachverlage GmbH 2006

Authors and Affiliations

  • M. L. Salazar Cavazos
    • 1
  • L. Y. Colunga González
    • 1
  • G. Gallegos de Lerma
    • 2
  • N. Waksman de Torres
    • 1
  1. 1.Departamento de Química AnalíticaU.A.N.L. Facultad de MedicinaMtyMéxico
  2. 2.Departamento de PatologíaU.A.N.L. Facultad de MedicinaMtyMéxico

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