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Simultaneous LC-MS-MS Analysis of Capecitabine and its Metabolites (5′-deoxy-5-fluorocytidine, 5′-deoxy-5-fluorouridine, 5-fluorouracil) After Off-Line SPE from Human Plasma

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Abstract

A new single extraction procedure was developed to isolate capecitabine and its major metabolites (5′-deoxy-5-fluorocytidine, 5′-deoxy-5-fluorouridine, and 5-fluorouracil) from human plasma. The simultaneous extraction of the four analytes was performed on an Atoll XWP solid phase support. Separation and detection were by liquid chromatography (5 µm Atlantis C18, 150 × 2.1 mm) and Turbospray Mass spectrometry in negative mode. To our knowledge, this report is the first to use these conditions for the simultaneous analysis of capecitabine and its metabolites.

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Authors and Affiliations

  1. Université Claude Bernard Lyon 1, UMR 5180, 43 Boulevard du 11 novembre 1918, 69622, Villeurbanne cedex, France

    A. Salvador

  2. CEPHAC Europe, 90 Avenue des Hauts de la Chaume, BP 28, 86281, Saint-Benoit, France

    A. Salvador, L. Millerioux & A. Renou

Authors
  1. A. Salvador
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  2. L. Millerioux
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  3. A. Renou
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Correspondence to A. Salvador.

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Salvador, A., Millerioux, L. & Renou, A. Simultaneous LC-MS-MS Analysis of Capecitabine and its Metabolites (5′-deoxy-5-fluorocytidine, 5′-deoxy-5-fluorouridine, 5-fluorouracil) After Off-Line SPE from Human Plasma. Chroma 63, 609–615 (2006). https://doi.org/10.1365/s10337-006-0799-5

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  • DOI: https://doi.org/10.1365/s10337-006-0799-5

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