Chromatographia

, Volume 63, Issue 9–10, pp 449–452 | Cite as

CZE Determination of Flavonoids in Halenia elliptica

Article

Abstract

A capillary zone electrophoretic method with UV detection at 254 nm has been developed and validated for quantitative analysis of three flavonoids, luteolin−7−OβD-glycoside, genkwanin, and luteolin, in Halenia elliptica. The applied voltage was 15 kV and the capillary temperature was kept constant at 25 °C. The effects on migration of buffer pH, electrolyte concentration, and organic modifier were studied systematically. Optimum separation was achieved with 20 mM borate buffer at pH 9.00 containing10% (v/v) acetonitrile. Regression equations revealed a good linear relationship between the peak area of each compound and its concentration. All correlation coefficients were 0.9999. The relative standard deviations of migration time and peak areas were, respectively, <1.58 and 5.02%(intra-day) and <1.69% and 5.36% (inter-day). The amounts of the three compounds in the different parts of H. elliptica (stems, leaves, and flowers) and in the extracts of the flowers obtained with different solvents (100% methanol and 50% and 75% methanol in water) were successfully determined with satisfactory repeatability and recovery.

Keywords

Capillary electrophoresis Pharmaceutical analysis Flavonoid Halenia elliptica Gentianaceae 

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Copyright information

© Friedr. Vieweg & Sohn/GWV Fachverlage GmbH 2006

Authors and Affiliations

  1. 1.Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical PhysicsChinese Academy of SciencesLanzhouP.R. China
  2. 2.The Graduate University of Chinese Academy of SciencesBeijingP.R. China

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