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Large-Scale Isolation and Purification of Scoparone from Herba artemisiae scopariae by High-Speed Counter-Current Chromatography

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Abstract

A preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol- water (1:1:0.45:1.55, v/v/v/v) was successfully performed to isolate scoparone (6,7-dimethoxycoumarin, 6,7-dimethylesculetin, 6,7-DME) from the plant of Herba artemisiae scopariae, a traditional Chinese medicine. 233.5 mg Scoparone with the purity of 96.8% (determined by HPLC) was obtained in one-step elution from 800 mg crude extract. The recovery of scoparone was 91.8%, and the chemical structure of this compound was identified by IR, MS, 1H NMR and 13C NMR spectrum.

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Authors and Affiliations

  1. Shanghai Institute of Materia Medica, Shanghai Institute for Biological Science, Chinese Academy of Sciences, Shanghai, 201203, China

    C. H. Ma, Z. L. Sun, Z. X. Li & C. G. Huang

  2. Center of Research and Development on Life Sciences and Enviromental Sciences, Harbin University of Commerce, Harbin, 150076, China

    W. Ke

  3. Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of Pharmacy, Second Military Medical University, No. 325 Guohe Road, Shanghai, 200433, China

    J. Y. Peng, X. Zhou & G. R. Fan

Authors
  1. C. H. Ma
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  2. W. Ke
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  3. Z. L. Sun
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  4. J. Y. Peng
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  5. Z. X. Li
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  6. X. Zhou
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  7. G. R. Fan
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  8. C. G. Huang
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Corresponding authors

Correspondence to G. R. Fan or C. G. Huang.

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Ma, C.H., Ke, W., Sun, Z.L. et al. Large-Scale Isolation and Purification of Scoparone from Herba artemisiae scopariae by High-Speed Counter-Current Chromatography. Chroma 64, 83–87 (2006). https://doi.org/10.1365/s10337-006-0789-7

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  • DOI: https://doi.org/10.1365/s10337-006-0789-7

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