, Volume 64, Issue 1–2, pp 83–87 | Cite as

Large-Scale Isolation and Purification of Scoparone from Herba artemisiae scopariae by High-Speed Counter-Current Chromatography

  • C. H. Ma
  • W. Ke
  • Z. L. Sun
  • J. Y. Peng
  • Z. X. Li
  • X. Zhou
  • G. R. Fan
  • C. G. Huang


A preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol- water (1:1:0.45:1.55, v/v/v/v) was successfully performed to isolate scoparone (6,7-dimethoxycoumarin, 6,7-dimethylesculetin, 6,7-DME) from the plant of Herba artemisiae scopariae, a traditional Chinese medicine. 233.5 mg Scoparone with the purity of 96.8% (determined by HPLC) was obtained in one-step elution from 800 mg crude extract. The recovery of scoparone was 91.8%, and the chemical structure of this compound was identified by IR, MS, 1H NMR and 13C NMR spectrum.


Column liquid chromatography Counter-current chromatography Coumarin compounds Scoparone Herba artemisiae scopariae Artemisia capillaries and Artemisia scoparia 


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Copyright information

© Friedr. Vieweg & Sohn/GWV Fachverlage GmbH 2006

Authors and Affiliations

  1. 1.Shanghai Institute of Materia Medica, Shanghai Institute for Biological ScienceChinese Academy of SciencesShanghaiChina
  2. 2.Center of Research and Development on Life Sciences and Enviromental SciencesHarbin University of CommerceHarbinChina
  3. 3.Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of PharmacySecond Military Medical UniversityShanghaiChina

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