Chromatographia

, Volume 64, Issue 1–2, pp 83–87 | Cite as

Large-Scale Isolation and Purification of Scoparone from Herba artemisiae scopariae by High-Speed Counter-Current Chromatography

Article

Abstract

A preparative high-speed counter-current chromatography with a two-phase solvent system composed of n-hexane-ethyl acetate-methanol- water (1:1:0.45:1.55, v/v/v/v) was successfully performed to isolate scoparone (6,7-dimethoxycoumarin, 6,7-dimethylesculetin, 6,7-DME) from the plant of Herba artemisiae scopariae, a traditional Chinese medicine. 233.5 mg Scoparone with the purity of 96.8% (determined by HPLC) was obtained in one-step elution from 800 mg crude extract. The recovery of scoparone was 91.8%, and the chemical structure of this compound was identified by IR, MS, 1H NMR and 13C NMR spectrum.

Keywords

Column liquid chromatography Counter-current chromatography Coumarin compounds Scoparone Herba artemisiae scopariae Artemisia capillaries and Artemisia scoparia 

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References

  1. The State Pharmacopoeia Commission of P. R. China (2000) In: Pharmacopoeia of the Peoples Republic of China (English Edition). Chemical Industry Press, BeijingGoogle Scholar
  2. Okuno I, Uchida K, Nakamura M, Sakurawi K (1988) Chem Pharm Bull 36:769–775Google Scholar
  3. Aburada M, Sasaki H, Harada M (1976) Yakugaku zasshi 96:147–153Google Scholar
  4. Yamahara J, Kobayashi G, Matsuda H, Katayama T, Fujimura H (1989) J Ethnopharmacol 26:129–136CrossRefGoogle Scholar
  5. Okada Y, Miyauchi N, Suzuki K, Kobayashi T, Tsutsui C, Mayuzumi K, Nishibe S, Okuyama T (1995) Chem Pharm Bull 43:1385–1387Google Scholar
  6. Jang S Il, Kim YJ, Kim HJ, Lee JC, Kim HY, Kim YC, Yun YG, Yu HH, You YO (Article in Press) Life SciencesGoogle Scholar
  7. Liu YJ, Li Z, Lin ZB (2001) Journal of China Medical University 30:12–13Google Scholar
  8. Zhao MY, Zhu Z, Li Z (2000) Journal of China Medical University 29:335–337Google Scholar
  9. Hoult JRS, Payá M (1996) Gen Pharmacol Vasc Syst 27:713–722CrossRefGoogle Scholar
  10. Ito Y (1986) CRC Rev Crit Anal Chem 17:65–143Google Scholar
  11. Jiang Y, Lu HT, Chen F (2004) J Chromatogr A 1033:183–186CrossRefGoogle Scholar
  12. Peng JY, Yang GJ, Fan FR, Wu YT (2005) J Chromatogr A 1092:235–240CrossRefGoogle Scholar
  13. Lei L, Yang FQ, Zhang TY, Tu PF, Wu JL, Ito Y (2001) J Chromatogr A 912:181–185CrossRefGoogle Scholar
  14. Chen L, Han YS, Yang FQ, Zhang TY (2001) J Chromatogr A 907:343–346CrossRefGoogle Scholar
  15. Peng JY, Fan GR, Wu YT (2005) J Chromatogr A 1091:89–93CrossRefGoogle Scholar
  16. Razdan TK, Qadri B, Harkar S, Waight ES (1987) Phytochemistry 26:2063–2069CrossRefGoogle Scholar
  17. Yamahara J, Kobayashi G, Matsuda H, Katayama T, Fujimura H (1989) J Ethnopharmacol 26:129–136CrossRefGoogle Scholar

Copyright information

© Friedr. Vieweg & Sohn/GWV Fachverlage GmbH 2006

Authors and Affiliations

  1. 1.Shanghai Institute of Materia Medica, Shanghai Institute for Biological ScienceChinese Academy of SciencesShanghaiChina
  2. 2.Center of Research and Development on Life Sciences and Enviromental SciencesHarbin University of CommerceHarbinChina
  3. 3.Shanghai Key Laboratory for Pharmaceutical Metabolite Research, School of PharmacySecond Military Medical UniversityShanghaiChina

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