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Chromatographia

, Volume 63, Issue 5–6, pp 283–287 | Cite as

HPLC with Diode-Array Detection for Determination of Leflunomide in Tablets

  • D. S. Miron
  • C. Soldattelli
  • E. E. S. Schapoval
Article

Abstract

We have developed and validated a simple HPLC method for analysis of leflunomide in tablets. Method conditions were determined by assay of a photodegraded sample of leflunomide. Optimum chromatographic performance was obtained with a C18 column and acetonitrile-water as mobile phase. Comparison of spectra recorded with a diode-array detector during elution of the leflunomide peak enabled determination of method specificity. The method is highly sensitive (detection limit 10 ng mL−1) and robust to deliberate variation of the conditions (RSD of peak area < 2.0%). Precision and accuracy were adequate over the concentration range 10 to 100 µg mL−1. These results show the proposed method is suitable for its intended use.

Keywords

Column liquid chromatography Diode-array detection Leflunomide 

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Copyright information

© Friedr. Vieweg & Sohn/GWV Fachverlage GmbH 2006

Authors and Affiliations

  • D. S. Miron
    • 1
  • C. Soldattelli
    • 1
    • 2
  • E. E. S. Schapoval
    • 1
    • 2
  1. 1.Programa de Pós-graduação em Ciências Farmacêuticas, Faculdade de FarmáciaUniversidade Federal do Rio Grande do SulPorto AlegreBrazil
  2. 2.Departamento de Produção e Controle de Medicamentos, Faculdade de FarmáciaUniversidade do Rio Grande do SulPorto AlegreBrazil

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