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GC and GC-MS Studies on the Essential Oil and Thiophenes from Tagetes patula L.

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Abstract

The production and the composition of the compounds (mono-, sesquiterpenes and acetylenic thiophenes) obtained by the steam distillation of Tagetes patula L. have been investigated. The volatile oil was produced by steam distillation. GC was carried out on three types of stationary phase using flame ionization and mass selective detection. Percentage data were calculated by the area normalization method with very good repeatability (RSD below 5%). Oils from flower-heads were rich in β-caryophyllene (53.5%) and the leaves contained terpinolene in high concentration (21.1%). The main volatile component of the hairy roots and intact roots was 5-(3-buten-1-ynyl)-2,2'-bithienyl (BBT) yielding 28.5% and 44.0% in the oils. Three new minor constituents were identified as α-gurjunene, β-caryophyllene and (E)-β-farnesene. A flash chromatographic method was developed for the isolation of thiophenes from a solvent extract of intact roots. The collected fractions were screened by TLC and analyzed by GC-MS. Three thiophene fractions were obtained containing BBT, 5-(4-acetoxy-1-butynyl)-2,2'-bithienyl (BBTOAc) and 5-(4-hydroxy-1-butynyl)-2,2'-bithienyl (BBTOH).

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Authors and Affiliations

  1. Department of Pharmacognosy, Semmelweis University, 1085, Budapest, Üllői str. 26, Hungary

    Sz. Szarka, É. Héthelyi, É. Lemberkovics, P. Bányai & É. Szőke

  2. Russian Academy of Sciences, Timiryazev Institute of Plant Physiology, Botanicheskaya str. 35, Moscow, 127276, Russia

    I. N. Kuzovkina

Authors
  1. Sz. Szarka
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  2. É. Héthelyi
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  3. É. Lemberkovics
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  4. I. N. Kuzovkina
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  5. P. Bányai
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  6. É. Szőke
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Corresponding author

Correspondence to É. Szőke.

Additional information

Presented at: 6th Balaton Symposium on High-Performance Separation Methods, Siófok, Hungary, September 7–9, 2005

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Szarka, S., Héthelyi, É., Lemberkovics, É. et al. GC and GC-MS Studies on the Essential Oil and Thiophenes from Tagetes patula L.. Chroma 63 (Suppl 13), S67–S73 (2006). https://doi.org/10.1365/s10337-006-0729-6

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  • DOI: https://doi.org/10.1365/s10337-006-0729-6

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