, Volume 65, Issue 3–4, pp 245–248 | Cite as

Fast Chromatographic Separation of Plasticizers on Thin Layers of an Inorganic Ion-Exchanger: Quantitative Determination of Di(2-ethylhexyl)phthalate

  • A. Mirzaie
  • A. Jamshidi
  • S. Waqif-Husain
Full Short Communication


Fast and selective separation of dimethyl phthalate, diethyl phthalate, dibutyl phthalate, di(2-ethylhexyl)phthalate (DEHP), benzyl butyl phthalate, diisodecyl phthalate, dimethyl adipate, diethyl adipate, di(2-ethylhexyl)adipate, triethyl citrate, tributyl citrate, tributyl acetyl citrate and n-butyl stearate have been developed on thin layers of inorganic ion-exchanger stannic silicate using a mixture of toluene + ethyl acetate (10:1, v/v) as mobile phase. The development distance and time were 12 cm and 25 min, respectively. Quantitative determination of DEHP was made at wavelength 280 nm by Camag TLC Scanner-3. Limit of quantitation for DEHP was 0.50 μg per zone while its limit of detection was 0.05 μg per zone.


Thin layer chromatography Ion-exchange TLC Di(2-ethylhexyl)phthalate Plasticizers Densitometry 


  1. 1.
    Coultate TP (2002) Food: the chemistry of its components. Royal Society of Chemistry, Cambridge, pp 357–368Google Scholar
  2. 2.
    Latini G, Felice CD, Verrotti A (2004) Reprod Toxicol 19:27–33CrossRefGoogle Scholar
  3. 3.
    Li X, Zeng Z, Chen Y, Xu Y (2004) Talanta 63:1013–1019CrossRefGoogle Scholar
  4. 4.
    Marcilla A, Garcia S, Garcia-Quesada JC (2004) J Anal Appl Pyrolysis 71:457–463CrossRefGoogle Scholar
  5. 5.
    Nalli S, Cooper DG, Nicell JA (2006) Sci Total Environ 366:286–294Google Scholar
  6. 6.
    Tsumura Y, Ishimitsu S, Kaihara A, Yoshii K, Tonogai Y (2002) J Health Sci 48:493–502CrossRefGoogle Scholar
  7. 7.
    Tsumura Y, Ishimitsu S, Saito I, Sakai H, Kobayashi Y, Tonogai Y (2001) Food Addit Contam 18:449–460CrossRefGoogle Scholar
  8. 8.
    Tsumura Y, Ishimitsu S, Nakamura Y, Yoshii K, Kaihara A, Tonogai Y (2001) Food Addit Contam 18:569–579CrossRefGoogle Scholar
  9. 9.
    Hileman B (2005) Chem Eng News 83:32–36Google Scholar
  10. 10.
    Lau O, Wong SK (2000) J Chromatogr A 882:255–270CrossRefGoogle Scholar
  11. 11.
    Swan SH, Main KM, Liu F, Stewert SL, Kruse RL, Calafat AM (2005) Environ Health Perspect 113:1056–1061CrossRefGoogle Scholar
  12. 12.
    Zygoura PD, Paleologos EK, Riganakos KA, Kontominas MG (2005) J Chromatogr A 1093(1–2):29–35CrossRefGoogle Scholar
  13. 13.
    Castillo M, Oubina A, Barcelo D (1998) Environ Sci Technol 32:2180–2185CrossRefGoogle Scholar
  14. 14.
    Sorensen LK (2006) Rapid Commun Mass Spectrom 20(7):1135–1143CrossRefGoogle Scholar
  15. 15.
    Fiala F, Steiner I, Kubesh K (2000) Dtsch Lebensm Rundsch 90:51–57Google Scholar
  16. 16.
    Chen H, Wang Y, Zhu R (2006) Se Pu 24(1):69–72Google Scholar
  17. 17.
    Gómez-Hens A, Aguilar-Caballos MP (2003) Trends Analyt Chem 22:847–857CrossRefGoogle Scholar
  18. 18.
    Qureshi M, Varshney KG (eds) (1991) Inorganic ion-exchanger in chemical analysis, CRC, Florida, p 1Google Scholar
  19. 19.
    Husain SW, Ghoulipour V, Sepahrian H (2004) Acta Chromatogr 14:102–109Google Scholar
  20. 20.
    Hassankhani-majd Z, Ghoulipour V, Husain SW (2006) Acta Chromatogr 16:173–180Google Scholar
  21. 21.
    Desai GT, Baxi DR (1978) Int J Technol 16:201–203Google Scholar
  22. 22.
    Husain SW, Ghoulipour V (1989) J Planar Chromatogr 2:474–476Google Scholar
  23. 23.
    Papp E, Farkas A, Otta KH, Mincsovics E (2000) J Planar Chromatogr 13:328–332Google Scholar
  24. 24.
    Ferenczi-Fodor R, Nagy-Turak A, Vegh Z (1995) J Planar Chromatogr 8:349–356Google Scholar
  25. 25.
    ICH Guideline Q2B (1996) Validation of analytical procedures: methodology (CPMP ICH/281/95), Geneva, SwitzerlandGoogle Scholar
  26. 26.
    Jamshidi A, Mobedi H, Ahmad-khanbeigi F (2006) J Planar Chromatogr 19:223–227Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2006

Authors and Affiliations

  1. 1.Faculty of Food Science and Technology, Science and Research BranchIslamic Azad UniversityTehranIran
  2. 2.Department of Novel Drug Delivery SystemsIran Polymer and Petrochemical InstituteTehranIran
  3. 3.Department of Applied Chemistry, Faculty of ChemistryUniversity of Tarbiat MoallemTehranIran

Personalised recommendations