, Volume 65, Issue 5–6, pp 367–371 | Cite as

Determination by LC of Polyphenols in Italian Red Wine

  • A. M. Tarola
  • V. Giannetti
Limited Short Communication


A reversed-phase liquid chromatographic method, optimised for the separation of trans-, and cis-resveratrol, catechin, epicatechin, quercetin and rutin, is reported. Separation was achieved using a C18 column and a gradient elution with acetonitrile and 5% formic acid aqueous solution. The analyses required an equilibration period of 10 min and a run time of 25 min for completion. Identification was based on retention characteristics and by relative UV spectra, obtained by photodiode array detector and were compared with commercial standards. Analyses were performed without any sample pre-treatment. Detection was carried out by UV–Vis detector at three different wavelengths. The detection limit ranged from 0.16 μgm L−1 (cis-resveratrol) to 1.5 μgm L−1 (+)-catechin. Investigation was extended to quantitative determination of phenol compounds in Italian red wine and to investigate the stability of the six antioxidants.


Column liquid chromatography Polyphenols Catechin and epicatechin Resveratrol Rutin and quercetin Red wine 


  1. 1.
    Goldberg DM, Hahn SE, Parkes JG (1995) Clin Chim Acta 237:155–187CrossRefGoogle Scholar
  2. 2.
    Criqui MH (1996) Clin Chim Acta 247:51–57CrossRefGoogle Scholar
  3. 3.
    Kannel WB, Ellison RC (1996) Clin Chim Acta 247:59–76CrossRefGoogle Scholar
  4. 4.
    Suh I, Shaten J, Cutler JA, Juller JA (1992) Ann Intern Med 116:881–887Google Scholar
  5. 5.
    Gaziano JM, Buring JE, Breslow JL, Goldhaber SZ, Rosner B, Van Denburgh M, Willet WN (1993) Engl J Med 329:1829–1834CrossRefGoogle Scholar
  6. 6.
    Jang MS, Cai E, Udeani GO, Slowing KV, Thomas CF, Beecher CWW, Fong HHS, Farnsworth NR, Kinghorn AD, Mehta RG, Moon RC, Pezzuto JM (1997) Science 275:218CrossRefGoogle Scholar
  7. 7.
    Clement MV, Hirpara JL, Chawdhury SH, Pervaiz S (1998) Blood 92:996–1002Google Scholar
  8. 8.
    El Attar TM, Virji AS (1999) Anticancer Drugs 10(2):187–193CrossRefGoogle Scholar
  9. 9.
    Pace-Asciak CR, Hahn S, Diamandis EP, Soleas G, Goldberg DM (1995) Clin Chim Acta 235:207–219CrossRefGoogle Scholar
  10. 10.
    Pinto MC, Gaecià-Barrado JA, Macias PJ Agric. (1999) Food Chem 47:4842–4846CrossRefGoogle Scholar
  11. 11.
    Langcake P, Pryce RJ (1976) Physiol Plant Phatol 9:77Google Scholar
  12. 12.
    Sato M, Suzuki Y, Okuda T, Yokotsuka K (1997) 61:1800–1805Google Scholar
  13. 13.
    Goldberg DM (1995) Clin Chem 41:14Google Scholar
  14. 14.
    Mattivi F, Reniero F, Korhammer SJ (1995) Agric Food Chem 43:1820CrossRefGoogle Scholar
  15. 15.
    Frankel EN, Waterhouse AL, Teissedre PL (1995) J Agric Food Chem 43:890–894CrossRefGoogle Scholar
  16. 16.
    Soleas G, Tomlinson G, Diamandis EP, Goldberg DM (1997) J Agric Food Chem 45:3995–4003CrossRefGoogle Scholar
  17. 17.
    Ghiselli A, Nardini M, Baldi A, Scaccini CJ (1998) Agric Food Chem 46(2):361–367CrossRefGoogle Scholar
  18. 18.
    Stavric B (1994) Clin Biochem 27:245–248CrossRefGoogle Scholar
  19. 19.
    Richter M, Ebermann R, Marian B (1999) Nutr Cancer 34(1):88–99CrossRefGoogle Scholar
  20. 20.
    Jeandet P, Bessis R, Maume BF, Sbaghi MJ (1993) Wine Res 4:79Google Scholar
  21. 21.
    Mayer AS, Heinonen M, Frankel EN (1998) Food Chem 61:71–75CrossRefGoogle Scholar
  22. 22.
    Chen CK, Pace-Asciak CR (1996) Gen Pharmacol 27:363–366Google Scholar
  23. 23.
    Alia M, Mateos R, Ramos S, Lecumberri E, Bravo L, Goya L (2006) Eur J Nutr 45:19–28Google Scholar
  24. 24.
    Gryglewski R, Korbut R, Robak J, Swies (1987) J Biochem Pharmacol 36:317–322CrossRefGoogle Scholar
  25. 25.
    Yutin C, Ronglian Z, Zhongjian J, Yong J (1990) Biol Med 9:19–21Google Scholar
  26. 26.
    Price SF, Breen PJ, Valladao M, Watson BT (1995) Am J Enol Vitic 46(2):187–194Google Scholar
  27. 27.
    Goldberg DM, Karumanchiri A, Tsang E, Soleas GJ Am. (1998) J Enol Vitic 49(1):23–34Google Scholar
  28. 28.
    Goldberg DM, Ng E, Karumanchiri A, Yan J, Diamandis EP, Soleas GJ (1995) J Chromatogr 708:85–89CrossRefGoogle Scholar
  29. 29.
    Snyder LR, Kirkland JJ, Glajch JL (1997) Practical HPLC method development. 2nd ed. Wiley, New YorkGoogle Scholar
  30. 30.
    Siemann EH, Creasy LL (1992) Am J Enol Vitic 43:49Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2007

Authors and Affiliations

  1. 1.Department of Control and Management of Commodities and Their Impact on EnvironmentUniversity “La Sapienza”RomeItaly

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