, Volume 65, Issue 3–4, pp 149–153 | Cite as

HPLC with Column Switching Coupled to APCI–MS for Pharmacokinetic Study of Amygdalin in Rabbit Plasma

  • Xinfeng Zhao
  • Weijin Zang
  • Xin Zhao
  • Shixiang Wang
  • Xiaohui Zheng
  • Jianbin Zheng


A fast and validated assay was established for the pharmacokinetic study of amygdalin in Armeniacae Semen in rabbit. The method involved column switching (CS) enrichment, separation, post-column derivatization, and atmospheric pressure chemical ionization (APCI) mass spectrometric detection. Plasma sample was enriched by CS using a MAYI-ODS as the first column. Analytes of interest were isolated and analyzed on a second column of Zorbax SB-C18. To detect amygdalin in plasma samples, a T-piece was connected between the HPLC outlet and the APCI source to add a mixture of dichloromethane and methanol to the eluent by an isocratic pump. Calibration graphs showed good linearity over a range of 1.0–1,280 ng mL−1. The detection limit was 0.2 ng mL−1. The intra- and inter-day accuracies were within 3.9%. The method was successfully applied to a study of the pharmacokinetics of amygdalin after an intravenous injection of amygdalin extracts to rabbits with a dose of 400 mg kg−1. The results indicate that amygdalin is a one-compartment open model with a first order absorption phase.


Column liquid chromatography Column switching APCI–MS detection Pharmacokinetics Amygdalin 



This work was supported by grants from the National Natural Science Foundation of China (No. 30470633, 30270554), External Intercourse and Cooperation Item (No. 30511120267), the Research Fund for the Doctoral Program (No.20050698012) and the Cultivation Fund of the Key Scientific and Technical Innovation Project, Ministry of Education of China (No.705045).


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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2006

Authors and Affiliations

  • Xinfeng Zhao
    • 1
    • 2
  • Weijin Zang
    • 1
  • Xin Zhao
    • 2
  • Shixiang Wang
    • 2
  • Xiaohui Zheng
    • 1
    • 2
  • Jianbin Zheng
    • 2
  1. 1.Department of Pharmacology, Key Laboratory of Environment and Genes Related to Diseases Ministry of Education, School of MedicineXi’an Jiaotong UniversityXi’anPeople’s Republic of China
  2. 2.Institute of Analytical Science/Shaanxi Provincial Key Laboratory of Elecetroanalytical ChemistryNorthwest UniversityXi’anPeople’s Republic of China

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