Chromatographia

, Volume 64, Issue 9–10, pp 577–581 | Cite as

Simultaneous Quantitation of Tetrahydropalmatine and Protopine in Rabbit Plasma by HPLC–PAD, and Application to Pharmacokinetic Studies

Short Communication

Abstract

A reversed-phase high-performance liquid chromatographic method coupled with photodiode-array detection (HPLC–PAD) has been developed and validated for simultaneous quantitation of dl-tetrahydropalmatine (dl-THP) and protopine (PTP), two active constituents of the herbal medicine Rhizoma Corydalis, in rabbit plasma. The pharmacokinetics of dl-THP and PTP were researched in rabbits after oral administration of extracts of Rhizoma Corydalis. Optimum separation was achieved on a reversed-phase column with methanol–phosphate buffer (pH 7.75, 65:35, v/v) as mobile phase. The experimental results showed the intra-day and inter-day precision and accuracy of the method were satisfactory for simultaneous determination of dl-THP and PTP at low, middle, and high concentrations. The lower limits of quantitation were 2.05 ng mL−1 for dl-THP and 2.10 ng mL−1 for PTP. Plasma concentration–time data for dl-THP were best fitted by the two-compartment linear pharmacokinetic model whereas data for PTP were best fitted by the one-compartment model.

Keywords

Column liquid chromatography Pharmacokinetics dl-Tetrahydropalmatine Protopine Rhizoma Corydalis 

Notes

Acknowledgements

This project was supported by the National Natural Science Foundation of China (No 20575039) and the Doctoral Spot Foundation of Chinese Ministry of Education (No 20050718011).

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Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2006

Authors and Affiliations

  1. 1.School of Chemistry and Materials ScienceShaanxi Normal UniversityShaanxiChina
  2. 2.Key Laboratory of Medicinal Plant Resource and Natural Pharmaceutical Chemistry of the Ministry of EducationShaanxi Normal UniversityShaanxiChina

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