, Volume 64, Issue 9–10, pp 577–581 | Cite as

Simultaneous Quantitation of Tetrahydropalmatine and Protopine in Rabbit Plasma by HPLC–PAD, and Application to Pharmacokinetic Studies

Short Communication


A reversed-phase high-performance liquid chromatographic method coupled with photodiode-array detection (HPLC–PAD) has been developed and validated for simultaneous quantitation of dl-tetrahydropalmatine (dl-THP) and protopine (PTP), two active constituents of the herbal medicine Rhizoma Corydalis, in rabbit plasma. The pharmacokinetics of dl-THP and PTP were researched in rabbits after oral administration of extracts of Rhizoma Corydalis. Optimum separation was achieved on a reversed-phase column with methanol–phosphate buffer (pH 7.75, 65:35, v/v) as mobile phase. The experimental results showed the intra-day and inter-day precision and accuracy of the method were satisfactory for simultaneous determination of dl-THP and PTP at low, middle, and high concentrations. The lower limits of quantitation were 2.05 ng mL−1 for dl-THP and 2.10 ng mL−1 for PTP. Plasma concentration–time data for dl-THP were best fitted by the two-compartment linear pharmacokinetic model whereas data for PTP were best fitted by the one-compartment model.


Column liquid chromatography Pharmacokinetics dl-Tetrahydropalmatine Protopine Rhizoma Corydalis 



This project was supported by the National Natural Science Foundation of China (No 20575039) and the Doctoral Spot Foundation of Chinese Ministry of Education (No 20050718011).


  1. 1.
    China Pharmacopoeia Committee (2000) Chinese Pharmacopoeia (Part I, 2000 edition), Chemical Industry Publishing House, Beijing, P.R. ChinaGoogle Scholar
  2. 2.
    Dhamanada S (1994) Herbal Gram 31:28–31Google Scholar
  3. 3.
    Leung WC, Zheng H, Huen M, Law SL, Xue H (2003) Prog Neuro-Psychopharmacol Biol Psychiatr 27:775–779CrossRefGoogle Scholar
  4. 4.
    Lin MT, Chueh FY, Hsieh MT, Chen CF, (1996) Clin Exp Pharmacol Physiol 23:738–745Google Scholar
  5. 5.
    Liu GG, Algeris A, Garattini S (1982) Arch Int Pharmacodyn Ther 258:39–50Google Scholar
  6. 6.
    Chang CK, Lin MT (2001) Neurosci Lett 307:163–166CrossRefGoogle Scholar
  7. 7.
    Ko FN, Wu TS, Lu ST, Wu YC, Huang TF, Teng CM (1989) Thromb Res 56:289–208CrossRefGoogle Scholar
  8. 8.
    Song LS, Ren GJ, Chen ZL, Chen ZH, Zhou ZN, Cheng H (2000) Br J Pharmacol 129:893–900CrossRefGoogle Scholar
  9. 9.
    Ustunes L, Laekeman GM, Gozler B, Vlietinck AJ, Ozer A, Herman AG (1988) J Nat Prod 51:1021–1022CrossRefGoogle Scholar
  10. 10.
    Saeedu SA, Gilani AH, Majoo RU, Shah BH (1997) Pharma Res 36:1–7CrossRefGoogle Scholar
  11. 11.
    Zhu M (1985) Chin J Pharm Anal 5:139–140Google Scholar
  12. 12.
    Fu XY (1985) Chin J Pharm Anal 5:194–196Google Scholar
  13. 13.
    Xing ZR (1990) Chin JMAP 7:18–19Google Scholar
  14. 14.
    Li YQ, Cui SY, Cheng YQ, Chen XG, Hu ZD (2004) Anal Chim Acta 508:17–22CrossRefGoogle Scholar
  15. 15.
    Büchele B, Zugmaier W, Genze F, Simmet T (2005) J Chromatogr B 829:144–148Google Scholar
  16. 16.
    Radwan MA, Abdine HH, Al-Quadeb BT, Aboul-Enein HY, Nakashima K (2006) J Chromatogr B 830:114–119CrossRefGoogle Scholar
  17. 17.
    You J, Cui F, Li Q, Yong-sheng W, Han X, Yu Y (2005) J Chromatogr B 823:172–176Google Scholar
  18. 18.
    Liao QF, Jia Y, Gao QT, Chen XH, Tan XJ, Bi KS (2005) Chromatographia 62:103–107CrossRefGoogle Scholar
  19. 19.
    Hong ZY, Fan GR, Chai YF, Yin XP, Wen J, Wu YT (2005) J Chromatogr B 826:108–113CrossRefGoogle Scholar
  20. 20.
    Xu MJ, Wang GJ, Xie HT, Wang R, Wang W, Li XY, Li H, Zhu DN, Yue L (2005) J Chromatogr B 828:55–61Google Scholar
  21. 21.
    Mathematical Pharmacology Professional Committee of China, (2005) Drug and statistics software (DAS), version 2.0Google Scholar
  22. 22.
    Oliveira EJ, Watson DG (2001) J Chromatogr B 764:3–25Google Scholar

Copyright information

© Friedr. Vieweg & Sohn Verlag/GWV Fachverlage GmbH 2006

Authors and Affiliations

  1. 1.School of Chemistry and Materials ScienceShaanxi Normal UniversityShaanxiChina
  2. 2.Key Laboratory of Medicinal Plant Resource and Natural Pharmaceutical Chemistry of the Ministry of EducationShaanxi Normal UniversityShaanxiChina

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