Abstract
A capillary zone electrophoresis method with HP-β-CD as chiral selector was established for the chiral separation of four stereoisomers of 1-(4-hydroxy-3-methoxy)-phenyl-2-[4-(1,2,3-trihydroxy-propyl)-2-methoxy]-phenoxy-1,3-propandiol for the first time, which were isolated from Hydnocarpus annamensis. The effects of chiral selector type and concentration, buffer composition, pH and concentration, and cartridge temperature on the enantioseparation were investigated. A baseline separation of the four stereoisomers was achieved in less than 18 min under the optimized conditions: 40 mmol L−1 Borax–NaOH buffer (pH 10.02) in the presence of 100 mmol L−1 HP-β-CD at 15°C and 30 kV. The experimental results showed that the method by capillary zone electrophoresis for the separation of four stereoisomers is powerful, sensitive and fast, requires less amounts of reagents, and can be employed as a reliable alternative to other methods.
References
Zhonghuabencao Editorial Board (1999) Zhonghuabencao. Shanghai Scientific and Technological Press, Shanghai 5:4479–4481
Shi HM, Liu Y, Jiang Y, Sun Z, Tu PF, Ling XM (2006) Chinese J Magn Reson (Revised)
You QD, Lin GQ (2004) Chrial drugs Research and application. In: Chamical Industry Press, Beijing
Lin XL, Zhu CF, Hao AY (2004) Anal Chim Acta 517:95–101
Chankvetadze B, Blaschke G (2001) J Chromatogr A 906:309–363
Gao WH, Kang JW (2006) J Chromatogr A 1108:145–148
Sheng LL, Lin CE (2004) J Chromatogr A 1032:213–218
Guo L, Lin SJ, Yang YF, Qi L, Wang MX, Chen Y (2003) J Chromatogr A 998:221–228
Yang GS, Chen DM, Chen Y, Yang Y, Tang B, Gao JJ, Aboul-Enein HY, Koppenhoefer B (2006) Chromatographia 62:441–445
Mateus L, Cherkaoui S, Christen P, Veuthey JL (2000) J Chromatogr A 868:285–294
Kvasnicka F, Biba B, Cvak L (2005) J Chromatogr A 1066:255–258
La S, Ahn S, Kim JH, Goto J, Choi OK, Kim KR (2002) Electrophoresis 23:4123–4131
María CP, Antonio LC, Marina ML (2006) Electrophoresis 27:887–895
Kang JW, Ou QY (1998) J Chromatogr A 795:394–398
Amini A (2001) Electrophoresis 22:3107–3130
Ward TJ (2002) Anal Chem 74:2863–2872
Schmitt U, Branch SK, Holzgrabe U (2002) J Sep Sci 25:959–974
Chankvetadze B (1997) Capillary electrophoresis in chiral analysis. Wileys, Chichester
Morante-Zarcero S, Crego AL, Sierra I, Fajardo M, Marina ML (2004) Electrophoresis 25:2745–2754
Rodríguez de Pablos R, García-Ruíz C, Crego AL, Marina ML (2005) Electrophoresis 26:1106–1113
Gomar AG, Ortega E, Calvet C, Merce R, Frigola J (2002) J Chromatogr A 950:257–270
Schmid MG, Wirnsberger K, Jira T, Bunke A, Gubitz G (1997) Chirality 9:153–156
Lin CE, Lin SL, Liao WS, Liu YC (2004) J Chromatogr A 1032:227–235
Jira T, Bunke A, Schmid MG, Gubitz G (1997) J Chromatogr A 761:269–275
Kavalirova A, Pospisilova M, Karlicek R (2004) Anal Chim Acta 525:43–51
Berzas Nevado JJ, Pe nalvo GC, Rodr ıguez Dorado RM (2005) Anal Chim Acta 533:127–133
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The work was financially supported by the National High-Tech Research and Development Program of China (863 Program) (2004AA2Z3783).
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Liu, Y., Shi, H., Sun, Z. et al. Enantiomer Separation of the Four Stereoisomers of 1-(4-Hydroxy-3-Methoxy)-Phenyl-2-[4-(1,2,3-Trihydroxy-Propyl)-2-Methoxy]-Phenoxy-1,3-Propandiol from Hydnocarpus annamensis by Capillary Zone Electrophoresis with HP-β-CD as Chiral Selector. Chroma 64, 483–486 (2006). https://doi.org/10.1365/s10337-006-0050-4
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DOI: https://doi.org/10.1365/s10337-006-0050-4