Chromatographia

, Volume 63, Issue 5–6, pp 249–253 | Cite as

High-Performance Liquid Chromatographic Determination of Isofraxidin in Rat Plasma

  • Q. Li
  • Y. Jia
  • L. -X. Sun
  • L. Xu
  • L. Tong
  • Z. -D. Shen
  • Y. -L. Liu
  • K. -S. Bi
Article

Abstract

For the first time a high-performance liquid chromatographic (HPLC) method, with liquid-liquid extraction and ultraviolet (UV) absorbance detection, has been developed for quantification of isofraxidin in rat plasma. The analysis was performed on a Diamonsil C18 column (200 mm × 4.6 mm i.d., 5 µm particle size) with acetonitrile–0.05% phosphoric acid, 26:74 (v/v), as isocratic mobile phase. The linear range was 0.05–8.0 µg mL−1 and the lower limit of quantification was 0.05 µg mL−1. The intra and inter-day relative standard deviation (RSD) for measurement of 0.25, 2.0, and 6.0 µg mL−1 quality-control (QC) samples ranged from 5.7 to 6.4% and from 6.3 to 7.9%, respectively. Accuracy, as relative error (RE), was from ±5.8% to ±7.3%. The method was validated for specificity, accuracy, and precision and was successfully used in a pharmacokinetic study of isofraxidin in rat plasma after administration of Ciwujia extract.

Keywords

Column liquid chromatography Pharmacokinetic study Isofraxidin Ciwujia extract Rat plasma 

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Copyright information

© Friedr. Vieweg & Sohn/GWV Fachverlage GmbH 2006

Authors and Affiliations

  • Q. Li
    • 1
  • Y. Jia
    • 1
  • L. -X. Sun
    • 1
  • L. Xu
    • 1
    • 2
  • L. Tong
    • 1
  • Z. -D. Shen
    • 1
  • Y. -L. Liu
    • 1
  • K. -S. Bi
    • 1
  1. 1.School of PharmacyShenyang Pharmaceutical UniversityShenyangP.R. China
  2. 2.The Analysis and Testing CenterInner Mongolia University for NationalitiesTongliaoP.R. China

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