Chromatographia

, Volume 63, Issue 1–2, pp 53–58 | Cite as

LC-ESI-MS Determination of Bilobalide and Ginkgolides in Canine Plasma

  • Jingling Tang
  • Jin Sun
  • Yinghua Sun
  • Fude Cui
  • Zhonggui He
Article

Abstract

A sensitive and selective method using liquid chromatography with electrospray ionization mass spectrometric detection was developed for the quantification of bilobalide and ginkgolides in canine plasma. The analytes were extracted with diethyl ether-dichloromethane-isopropanol (6:3:1, v/v) after spiking the samples with daidzein (internal standard). The lower limit of quantification (LLOQ) of the method was 2.5 µg L−1 for ginkgolide B and 10.0 µg L−1 for bilabolide, ginkgolide A and ginkgolide C. The accuracy of the method was within 15% of the actual values over a wide range of plasma concentrations. The intra-day and inter-day precision was better than 15% (R.S.D.). Finally, the LC-ESI-MS method was successfully applied to study the pharmacokinetics of ginkgolides and bilabolide after administration of Ginkgo biloba extracts to dogs.

Keywords

Column liquid chromatography LC-ESI-MS Pharmacokinetics Bilabolide and ginkgolides in canine plasma 

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Copyright information

© Friedr. Vieweg & Sohn/GWV Fachverlage GmbH 2005

Authors and Affiliations

  • Jingling Tang
    • 1
  • Jin Sun
    • 1
  • Yinghua Sun
    • 1
  • Fude Cui
    • 1
  • Zhonggui He
    • 1
  1. 1.Department of Biopharmaceutics, School of PharmacyShenyang Pharmaceutical UniversityShenyangChina

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