Chromatographia

, 62:649 | Cite as

Simultaneous Determination of Quercetin, Rutin and Coumaric Acid in Flowers of Rhododendron arboreum by HPTLC1

Article

Abstract

A simple and fast method was developed for simultaneous quantitative determination of three biologically active phenolic compounds i.e. quercetin, rutin and coumaric acid in flowers of Rhododendron arboreum using high-performance thin-layer chromatography (HPTLC). The separation was performed on TLC aluminium plates precoated with silica gel RP-18 F254S. Good separation was achieved in the mobile phase of methanol-water-formic acid (40:57:3, v/v/v) and densitometric determination of these compounds was carried out at 280 nm in reflectance/absorbance mode. The linear regression data for the calibration plots showed a good linear relationship with r = 0.9971, 0.9953 and 0.9960 for quercetin, rutin and coumaric acid, respectively. Accuracy of the method was checked by recovery study conducted at two different levels with the average recovery of 99.90%, 99.02% and 99.16% for quercetin, rutin and coumaric acid, respectively. The present method is being reported first time and may be used for routine quality control of the flowers of R. arboreum.

Keywords

Thin-layer chromatography Rhododendron arboreum Quercetin, rutin and coumaric acid 

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Copyright information

© Friedr. Vieweg & Sohn/GWV Fachverlage GmbH 2005

Authors and Affiliations

  • A. Swaroop
    • 1
  • A. Prakash Gupta
    • 1
  • A. Kumar Sinha
    • 1
  1. 1.Natural Plant Products DivisionInstitute of Himalayan Bioresource TechnologyPalampurIndia

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