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Chromatographia

, Volume 62, Issue 1–2, pp 41–48 | Cite as

Comparison of Different Clean-Up Procedures for the Analysis of Deoxynivalenol in Cereal-Based Food and Validation of a Reliable HPLC Method

  • M. Klötzel
  • S. Schmidt
  • U. Lauber
  • G. Thielert
  • H. -U. Humpf
Article

Abstract

Three different clean-up methods for the analysis of deoxynivalenol in cereals and cereal-based foods are described and compared. DON was extracted with water or acetonitrile/water (84/16, v/v) and the extracts were cleaned-up by passing either through two different immunoaffinity columns (DONtest, Vicam; DONPrep, Rhone) or a charcoal/alumina column (MycoSep, Romer Labs). Determinative analysis was performed by high performance liquid chromatography (HPLC) with diode array detection (DAD) and fluorescenic detection (FLD) after post-column derivatisation. Different parameters were optimised and possible reasons for diverse results are discussed. The final method was validated and adopted to different matrices. Best results were obtained using immunoaffinity columns for clean-up in combination with HPLC-FLD detection of the DON-derivative.

Keywords

Column liquid chromatography Sample clean-up procedures Deoxynivalenol Cereal-based foodstuff 

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Copyright information

© Friedr. Vieweg & Sohn/GWV Fachverlage GmbH 2005

Authors and Affiliations

  • M. Klötzel
    • 1
  • S. Schmidt
    • 2
  • U. Lauber
    • 1
  • G. Thielert
    • 2
  • H. -U. Humpf
    • 3
  1. 1.Chemisches und Veterinäruntersuchungsamt StuttgartFellbachGermany
  2. 2.Chemisches und Veterinäruntersuchungsamt SigmaringenSigmaringenGermany
  3. 3.Institut für LebensmittelchemieWestfälische Wilhelms-Universität MünsterMünsterGermany

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