Chromatographia

, Volume 61, Issue 11–12, pp 539–547 | Cite as

Oil-In-Water Microemulsion LC Determination of Pharmaceuticals Using Gradient Elution

Article

Abstract

Efficient and novel oil-in-water microemulsion HPLC (MELC) separations of a range of solutes have been performed on conventional reversed-phase HPLC columns using gradient elution. This work follows previous successful separations using isocratic oil-in-water MELC [1]. It was found that by changing certain variables, peak-peak resolution, separation selectivity, efficiency and solute retention could be manipulated. The method was compatible with very low UV detection wavelengths. A robust separation method was developed for the quantitative analysis of 2 steroids in a combination-inhaled product for asthma. The method offered similar chromatography and run time when compared with conventional HPLC modes, thus demonstrating its potential for routine use. Stability-indicating methods were developed to separate synthetic and degradative impurities from the main component peaks in 4 pharmaceutical products. The methods offered quicker analysis times and equivalent selectivity to conventional HPLC modes. In developing the separations the effect on the chromatography of varying the operating parameters was studied.

Keywords

Column liquid chromatography Micellar liquid chromatography Microemulsion liquid chromatography Pharmaceutical analysis Selectivity investigation 

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Copyright information

© Friedr. Vieweg & Sohn/GWV Fachverlage GmbH 2005

Authors and Affiliations

  1. 1.GlaxoSmithKline Research & DevelopmentNew Frontiers Science Park SouthEssexUK
  2. 2.Institute of Pharmaceutical Innovation, School of Life SciencesUniversity of BradfordUK

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