Abstract
A simple method is proposed for the simultaneous analysis of the neurotoxin β-N-oxalyl-α, β-diaminopropionic acid (β-ODAP) and its isomer α-ODAP, as well as other free amino acids, in Lathyrus sativus extract based on reversed-phase (C18 column) high-performance liquid chromatography (HPLC) of their para-nitro-benzyloxycarbonyl chloride (PNZ-Cl) derivatives. Samples were derivatized in 0.5 M NaHCO3 within 2 min at ambient temperature by adding 50 mM PNZ-Cl in acetonitrile. The PNZ-derivatives were quantified by their UV-absorption at 260 nm. Detection limits were approximately 8 ng mL−1 for both α-, β-isomers, 9 ng mL−1 for homoarginine at a signal-to-noise ratio of 3:1. The coefficients of determination were 0.9967–0.9991. The method was validated by the determination of β-, α-ODAP and homoarginine contents in ethanol-water (30:70, v/v) extracts from the seed and foliage of Lathyrus sativus.
Similar content being viewed by others
References
Swain T (1977) Annu Rev Plant Physiol 28:479–501
Lea PJ, Norris RD (1976) Phytochemistry 15:585–595
Rubenstein E (2000) Medicine 79:80–85
Smartt J., in Pulses of the Classical World, ed. Summerfield RJ, Ellis EH Cambridge Univ Press Cambridge 1990, pp. 190–200.
Lambein F, Haque A, Lathyrus sativus and human lathyrism, progress and prospects, 2nd International colloquium on Lathyrism in Bangladesh, Dhaka, 1993, pp. 1–53.
Campbell CG, Mehra RB, Agrawal SK, Chen YZ, Moneim A, Khawaja ANHIT, Yadov CR, Tay JU and Araya WA, in Current status and future strategy in breeding grasspea (Lathyrus sativus L.), ed. F.J. Muehlbauer and W.J. Kaiser, Kluwer Academic Publishers, Dordrecht, Netherlands, 1994, pp. 617–630.
Croft AM, Pang ECK, Taylor PWJ (1999) Euphytica 107:167–176
Rao SLN, Adiga PR, Saima PS (1964) Biochemistry 3:432–436
Ludolph AC, Hugon J, Dwivedi MP, Schaumburg HH, Spencer PS (1987) Brain 110:149–165
Tekle-Haimanot R, Abegaz BM, Wuhib E, Kassina A, Kidane Y, Kebede N, Alemu T, Spencer PS (1993) Nutrition Research 13:1113–1126
Murti VVS, Seshadri TR (1964) Current Sci (India) 33:323–329
Kuo YH, Ikegami F, Lambein F (1998) Phytochemistry 49:43–48
Paleacu D, Cohn DF, Rabey JM (1999) Parkinsonism and Related Disorders, 5:55–58
Hanbury CD, White CL, Mullan BP, Siddique KHM (2000) Animal Feed Science and Technology 87:1–27.
Chen YZ, Li ZX, Lü FH, Bao XG, Liu SZ, Liu XC, Zhang GW, Li YR (1992) J Lanzhou Univ (Natural Sciences, in Chinese) 28:89–92
Rao SLN (1978) Anal Biochem 86:386–395
Euerby MR, Nunn PB, Partridge LZ (1989) J Chromatogr 466:407–414
Kisby GE, Roy DN, Spencer PS (1988) J Neurosci Methods 26:45–54
Geda A, Briggs CJ, Venkataram S (1993) J Chromatogr 635:338–341
Chen X, Wang F, Chen Q, Qin XC, Li ZX (2000) J Agric Food Chem 48:3383–3386
Wang F, Chen X, Chen Q, Qin XC, Li ZX (2000) J Chromatogr A 883:113–118
Khan JK, Kuo YH, Kebede N, Lambein F (1994) J Chromatogr A 687:113–119
Brückner H, Lüpke M (1995) J Chromatogr A 697:295–307
Li ZX., Meng YF, Zhang L, Chen YZ (1992) J Lanzhou Univ (in Chinese) 28:89–92
Li YL, Pan XF, Huang WK (1975) J Lanzhou Univ (in Chinese) 2:1–4
Roy DN, Rao BSN (1968) Current Science (India) 37:395–396
Wu G, Bowlus SB, Kim KS, Haskell BE (1976) Phytochemistry 15:1257–1259
Chase RA, Pearson S, Nunn PB, Lantos PL (1985) Neurosci Lett 55:89–94
Bell EA, O’Donovan JP (1966) Phytochemistry 15:1211–1219
Bruyn AD, Becu C, Lambein F, Kebede N, Abegaz BM, Nunn PB (1994) Phytochemistry 36:85–89
Lambein F (2000) Lathyrus & Lathyrism Newsletter 1:4–5
Bell EA (2003) J Agric Food Chem 51:2854–2865
Acknowledgments.
This work was supported by NKBRSF Project (G2000018603) and the National Natural Science Foundation of China (No. 30270965). Dr. Peter S. Spencer who is from Oregon Health and Science University, Portland, USA, is thanked for assistance with the manuscript.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yan, Z., Wang, Y., Jiao, C. et al. High-Performance Liquid Chromatographic Analysis of Neurotoxin β-N-Oxalyl-α, β-diaminopropionic Acid (β-ODAP), Its Non-neurotoxic Isomer α-ODAP and Other Free Amino Acids in Lathyrus sativus. Chroma 61, 231–236 (2005). https://doi.org/10.1365/s10337-005-0500-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-005-0500-4