Abstract
Capillary electrochromatography (CEC) has been performed with a series of C14, methlyacrylamide based monolithic columns. These monoliths with different porosities were prepared by in-situ copolymerization in fused-silica capillaries. The porous properties of monoliths were further observed using scanning electron micrographs (SEM) and measured using a mercury porosimeter. The effect of various alcohols as porogens on porous structural properties and chromatographic behaviors were also investigated. The effects of organic additive, pH value and ionic strength in mobile phase on electroosmosis flow (EOF) and separation were further discussed. Meanwhile, the baseline separation of 6 neutral compounds can be well obtained. In addition, the monolithic column demonstrates the high column efficiency and satisfactory reproducibility.
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Acknowledgements We gratefully acknowledge the support of the National Natural Science Foundation of China.
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Zhang, K., Gao, R., Yan, C. et al. Preparation and Porous Property of C14-Monolithic Column for Capillary Electrochromatography. Chromatographia 61, 55–60 (2005). https://doi.org/10.1365/s10337-004-0464-9
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DOI: https://doi.org/10.1365/s10337-004-0464-9