Abstract
A method for the determination of iodate was developed by reversed-phase high-performance liquid chromatography with UV detection. Iodate was converted to iodine, which was separated from the matrix using a reversed-phase Ultrasphere C18 column (250 × 4.6 mm, 5 μm) with methanol-1 mmol L−1 H3PO4 (20:80, v/v) as mobile phase at 1.00 mL min−1 and UV detection at 224 nm. The calibration graph was linear from 0.05 μg mL−1 to 5.00μg mL−1 for iodine with a correlation coefficient of 0.9994 (n=7). The detection limit was 0.01 μg mL−1. The method was successfully applied to the determination of iodate in iodized salt. The recovery was from 96% to 101% and the relative standard deviation was in the range of 1.5% to 2.9%.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Li HB, Chen F, Xu XR (2001) J Chromatogr A 918:335–339
Underwood EJ (1997) Trace Elements in Human and Animal Nutrition, 4th Edn., Academic Press, New York, pp. 271–275
Yao SZ, Chen P, Wei WZ (1999) Food Chem 67:311–316
Barzegar M, Khajehsharifi H, Mousavi MF (2003) Can J Anal Sci Spect 48:303–307
Afkhami A, Mosaed F (2002) Anal Sci 18:667–670
Ma WX (1999) Spectrosc Spect Anal 19:763–764
Gosain S, Pandey M, Verma KK (1999) J AOAC Int 82:167–171
Silva RL, de Oliveira AF, Neves EA (1998) J Brazil Chem Soc 9:171–174
Choengchan N, Uraisin K, Choden K, Veerasaia W, Grudpan K, Nacaprich D (2002) Talanta 58:1195–1201
Jakmunee J, Grudpan K (2001) Anal Chim Acta 438:299–304
Kosminsky L, Bertotti M (1999) Electroanal 11:623–626
Kumar SD, Maiti B, Mathur PK (2001) Talanta 53:701–705
Fu HT, Zhao LM, Zhang YL (1999) Chin J Anal Chem 27:684–686
Das P, Gupta M, Jain A, Verma KK (2004) J Chromatogr A 1023:33–39
Zhao Y (2000) Chromatographia 51:231–234
Barroso MJ, San Andres MP, Vera S (2000) Chromatographia 51:277–282
Vachirapatama N, Dicinoski GW, Townsend AT, Haddad PR (2002) J Chromatogr A 956:221–227
Hu QF, Yang GY, Yin JY, Yao Y (2002) Talanta 57:751–755
Paull B, Twohill E, Bashir W (2000) J Chromatogr A 877:123–132
Sanchez JM, Obrezkov O, Salvado V (2000) J Chromatogr A 871:217–226
Vachirapatama N, Macka M, Paull B, Munker C, Haddad PR (1999) J Chromatogr A 850:257–268
Oszwaldowski S, Lipka R, Majewski T, Jarosz M (1998) Analyst 123:1529–1533
Wang H, Zhang HS, Cheng JK, Qiu PH (1997) Microchem J 55:332–339
Xu XR, Li HB, Gu JD, Paeng KJ (2004) Chromatographia 59: 745–747
Swartz ME, Krull IS (1997) Analytical Method Development and Validation. Marcel Dekker, New York, pp. 53–71
Li HB, Chen F, Jiang Y (2000) J Chromatogr A 891:243–247
Huang XZ (1987) Iodine. In: Cheng GZ, Huang XZ, Liu WY (eds) Ultra-Visible Spectrophotometric Methods, Vol. 2. Atomic Energy Press, Beijing, pp. 201–210
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Xu, X., Li, H., Gu, JD. et al. Determination of Iodate in Iodized Salt by Reversed-Phase High-Performance Liquid Chromatography with UV Detection. Chromatographia 60, 721–723 (2004). https://doi.org/10.1365/s10337-004-0430-6
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-004-0430-6