Abstract
A flow-electrolytic cell containing a strand of carbon fibers has been designed and characterized for use in a voltammetric detector for high-performance liquid chromatography. The detector was used for determination of triclosan (2,4,4′-trichloro-2′-hydroxydiphenyl ether) in rabbit serum and urine. Analysis of rabbit serum and urine 1 day and 1 to 5 days, respectively, after ingestion of oral triclosan revealed that the concentration of triclosan was higher than for control serum and urine. The concentration reached maximum levels after 6 h and 34 h or 44 h in serum and urine, respectively. When triclosan was determined in rabbit samples with the method proposed the results obtained were comparable with those obtained by high-performance liquid chromatography with ultraviolet detection.
Similar content being viewed by others
References
Patonay G (1992) HPLC Detection Newer Methods, Wiley-VCH, p 98
Wang J (1999) Electroanalytical Techniques in Clinical Chemistry and Laboratory Medicine, Wiley-VCH, p114
Parves H, Bastart-Malsory M, Parvez S, Nagatsu T, Garpentier G (1987) Electrochemical Detection in Medicine and Chemistry, VNU Science Press, p 142
Besenhard JO, Schulte A, Schur K, Jannakoudakis PD (1991) Preparation of Voltammetric and Potentiometric Carbon Fiber Microelectrodes, NATO ASI Ser, Ser E 189–204
Fernandez ALS, Calzon JAG, Garcia AC, Blanco PT (1991) Electroanalysis 43:413–417
Hernandez P, Sanchez I, Paton F, Hernandez L (1998) Talanta 46:985–991
Alarnes-Varela G, Suarez-Fernandez AL, Costa-Garcia A (1998) Electrochim Acta 44:763–772
Wang JS, Cai XA, Fernandes JR, Grant DH, Ozsoz MJ (1998) Electroanal Chem 441:167–172
Suarez-Fernandez AL, Alarnes-Varela G, Costa-Garcia A (1999) Electrochim Acta 44:4489–4498
Cavalheiro ETG, Brajter-Toth A (1999) J Pharm Biomed Anal 19:217–230
Logman MJ, Budygain EA, Gainetdinov RR, Mark RJ (2000) Neurosci Methods 95:95–102
Gonzalez de la Huabra MJ, Hernandez P, Ballesteros Y, Hernandez L (2001) Talanta 54:1077–1085
Jenkins S, Addy M, Nowcombe RJ (1993) J Clin Periodontol 20:609–612
Kanetoshi A, Katsura E, Ogawa H, Uhyama T, Kaneshima H, Miura T (1992) Arch Environ Contam Toxicol 23:91–98
Aguera A, Fernandes-Alba AR, Piedra L, Mezcua M, Gomez MJ (2003) Anal Chim Acta 480:193–205
Pemberton RM, Hart JP (1999) Anal Chim Acta 390:107–115
Safavi A, Maleki N, Shahbaazi HR (2003) Anal Chim Acta 494:225–233
Akiok K, Hiroshi O, Eijik, Toyo O, Hiroyasuk K (1998) Arch Environ Contam Toxicol 17:637–644
Acknowledgments.
Financial support of this work by National Science Council of the Republic of China (no. NSC 90-2113-M-041-006) is gratefully acknowledged.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wang, LH., Chu, SC. Voltammetric Detector for Liquid Chromatography: Determination of Triclosan in Rabbit Urine and Serum. Chromatographia 60, 385–390 (2004). https://doi.org/10.1365/s10337-004-0414-6
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1365/s10337-004-0414-6