Voltammetric Detector for Liquid Chromatography: Determination of Triclosan in Rabbit Urine and Serum
A flow-electrolytic cell containing a strand of carbon fibers has been designed and characterized for use in a voltammetric detector for high-performance liquid chromatography. The detector was used for determination of triclosan (2,4,4′-trichloro-2′-hydroxydiphenyl ether) in rabbit serum and urine. Analysis of rabbit serum and urine 1 day and 1 to 5 days, respectively, after ingestion of oral triclosan revealed that the concentration of triclosan was higher than for control serum and urine. The concentration reached maximum levels after 6 h and 34 h or 44 h in serum and urine, respectively. When triclosan was determined in rabbit samples with the method proposed the results obtained were comparable with those obtained by high-performance liquid chromatography with ultraviolet detection.
KeywordsColumn liquid chromatography Voltammetric detector Carbon-fiber electrode Rabbit urine and serum Triclosan
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Financial support of this work by National Science Council of the Republic of China (no. NSC 90-2113-M-041-006) is gratefully acknowledged.
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