, Volume 60, Issue 7–8, pp 399–404 | Cite as

Rapid Screening of Drug Absorption Potential Using the Immobilized Artificial Membrane Phosphatidylcholine Column and Molar Volume

  • C. H. Yoon
  • B. S. Shin
  • H. S. Chang
  • L. S. Kwon
  • H. Y. Kim
  • S. E. Yoo
  • S. D. Yoo


The immobilized artificial membrane phosphatidylcholine (IAMPC) chromatography was evaluated for the predictability of oral absorption potential of 40 structurally unrelated drugs. The chromatographic capacity factors (kIAM) were determined as a function the pH and composition of the mobile phase, and were corrected for the molar volume of the solutes (kIAM/MW n ). The correlation between kIAM/MW n and the human fraction of intestinal absorption (Fa) was highest when measured at 20% acetonitrile (pH 5.5) with the power function n = 2.5. The best-fit equation for the sigmoid relationship between kIAM/MW n and Fa was obtained: Fa (%) = 94.3 × {1-exp[-17.9 × (kIAM/MW2.5) × 106]}^2.1 (r = 0.925). This in vitro prediction method may be useful in a rapid screening of drug candidates with high oral absorption potential in humans.


Column liquid chromatography Immobilized artificial membrane Oral absorption potential High throughput screening Molar volume 


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This work was supported by the Century 21 Frontier Research Program Grant CBM-C800-001 from the Ministry of Science and Technology, Korea. Seok Chun Scholarship for SDY from Sungkyunkwan University is appreciated.


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Copyright information

© Friedr. Vieweg&Sohn/GWV Fachverlage GmbH 2004

Authors and Affiliations

  • C. H. Yoon
    • 1
  • B. S. Shin
    • 1
  • H. S. Chang
    • 1
  • L. S. Kwon
    • 2
  • H. Y. Kim
    • 2
  • S. E. Yoo
    • 3
  • S. D. Yoo
    • 1
  1. 1.College of PharmacySungkyunkwan UniversitySuwonKorea
  2. 2.C&C Research LaboratoriesHwasung-siKorea
  3. 3.The Center for Biological Modulators Korea Research Institute of Chemical TechnologyYuseong-guKorea

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