Adsorption Isotherms and Sites Distribution of Caffeic Acid – Imprinted Polymer Monolith from Frontal Analysis
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The frontal analysis technique was successfully applied to the determination of the adsorption isotherms of the imprinted polymer monolith prepared by a in-situ synthesis method using caffeic acid as the template, and methacrylic acid, ethyl glycol dimethacrylate and2,2’-azobisisobutyronitrile as the functional monomer, cross-linker and initiator, respectively, with tetrahydrofuran-iso-octane (2:1, ν/ν) as a porogen. Adsorption isotherms of the monolith were measured at 25, 45 and 60°C using tetrahydrofuran containing 1, 3 and 5% acetic acid as mobile phase, respectively. The adsorption capacity of the monolith for the template was found to be much stronger than the other structurally related compounds. The column temperature significantly affected the adsorption capacity of the monolith. The acetic acid in the mobile phase showed some influence on the adsorption of the template on the stationary phase. The adsorption behavior of the template on the monolith is best described by the Freundlich isotherm. From the best coefficients of the Bi-Langmuir fit, the binding constant and saturation capacities of the molecularly imprinted polymer monolith were obtained.
KeywordsColumn liquid chromatography Imprinted polymer monolith Frontal analysis Adsorption isotherms Caffeic acid
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Financial supports from China NSF, China MST and Hunan Provincial Department of Science & Technology were acknowledged.
- Svec F, Frechet JMJ (1992) Anal Chem 64:820–822Google Scholar
- Matsui J, Kato T, Takeuchi T, Suzuki M, Yokoyama K, Tamiya E, Karube I (1993) Anal Chem 65:2223–2224Google Scholar
- Yamamura HL, Enna SJ, Kuhar MJ (1985) Neurotransmitter Receptor Binding, Raven press, New YorkGoogle Scholar
- Matsui J, Miyoshi Y, Doblhoff-Dier O, Takeuchi T (1995) Anal Chem 67:4404–4408Google Scholar
- Bogacheva EK, Kiselev AV, Yu AV, Eltekov A (1964) Kollid Zh 26:456–459Google Scholar
- Guiochon G, Golshan-Shirazi S, Katti A (1994) Foundamentals of preparative and nonlinear chromatography, Academic Press, New WorkGoogle Scholar
- Aris R, Amundson NR (1973) Mathematical method in chemical engineering, New York, Prentice-Hall, Englewood CliffsGoogle Scholar
- Jaroniec M, Madey R (1988) Physical adsorption on heterogeneous solids, Elsevier, AmsterdamGoogle Scholar
- Rudzinske W, Everett, DH (1992) Adsorption of gases on the heterogeneous surface, Academic Press, New YorkGoogle Scholar