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Chromatographia

, Volume 60, Issue 7–8, pp 425–431 | Cite as

Adsorption Isotherms and Sites Distribution of Caffeic Acid – Imprinted Polymer Monolith from Frontal Analysis

  • Hui Li
  • Lihua Nie
  • Shouzhuo Yao
Article

Abstract

The frontal analysis technique was successfully applied to the determination of the adsorption isotherms of the imprinted polymer monolith prepared by a in-situ synthesis method using caffeic acid as the template, and methacrylic acid, ethyl glycol dimethacrylate and2,2’-azobisisobutyronitrile as the functional monomer, cross-linker and initiator, respectively, with tetrahydrofuran-iso-octane (2:1, ν/ν) as a porogen. Adsorption isotherms of the monolith were measured at 25, 45 and 60°C using tetrahydrofuran containing 1, 3 and 5% acetic acid as mobile phase, respectively. The adsorption capacity of the monolith for the template was found to be much stronger than the other structurally related compounds. The column temperature significantly affected the adsorption capacity of the monolith. The acetic acid in the mobile phase showed some influence on the adsorption of the template on the stationary phase. The adsorption behavior of the template on the monolith is best described by the Freundlich isotherm. From the best coefficients of the Bi-Langmuir fit, the binding constant and saturation capacities of the molecularly imprinted polymer monolith were obtained.

Keywords

Column liquid chromatography Imprinted polymer monolith Frontal analysis Adsorption isotherms Caffeic acid 

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Notes

Acknowledgments.

Financial supports from China NSF, China MST and Hunan Provincial Department of Science & Technology were acknowledged.

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Copyright information

© Friedr. Vieweg&Sohn/GWV Fachverlage GmbH 2004

Authors and Affiliations

  1. 1.State Key Laboratory of Chemo/Biological Sensing & ChemometricsHunan UniversityChangshaP.R. China
  2. 2.College of Chemistry and Chemical EngineeringJishou UniversityHunan JishouP.R. China

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