Validated Method for Tadalafil Analysis in Pharmaceutical Preparations by Capillary Electrophoresis
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A simple, rapid and inexpensive capillary electrophoretic method has been developed and validated for the determination of tadalafil in pharmaceutical preparations. The analysis was carried out using a fused silica capillary (60 cm × 75 μm I.D.), phosphate buffer (50 mM, 3.0 pH) as back ground electrolyte (BGE), 15 kV applied voltage with UV detection at 254 nm and at a working temperature of 23 ± 1 °C. Linearity was observed in the concentration range from 200–5000 μg/mL, with a correlation coefficient (R2) of 0.9998 and 200 μg/mL as the limit of detection. The percentage recovery of tadalafil from pharmaceutical preparations was 99.5. Validation parameters prove the precision of the method and its applicability for the determination of tadalafil in pharmaceutical tablet formulations. The method is fast and is suitable for high throughput analysis of the drug.
KeywordsCapillary electrophoresis Tadalafil Pharmaceutical tablet formulation
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- Tadalafil, New Product Evaluation, Trent Regional Medicines Information Service, Leicester Royal Infirmary, Leicester LE1 5WW, 2003Google Scholar
- Aboul-Enein HY, Ali I (2004) Capillary Electrophoresis, in Analytical Instrumentation hand book, Cazes of (ed) Marcel Dekker, Inc., New York, (In Press)Google Scholar
- The United State Pharmacopeia, 24th Ed.; United States Pharmacopeial Convention Rockville, MD, (2000) 2150–2151Google Scholar
- Miller JC, Miller JN (1984) Statistics for analytical chemistry, Wiley, New YorkGoogle Scholar