Chromatographia

, Volume 60, Issue 1–2, pp 125–130 | Cite as

Determination and Differentiation of Two Seemingly Identical Medicinal Herbs by Capillary Electrophoresis with Electrochemical Detection

Article

Abstract

A high-performance capillary electrophoresis with electrochemical detection (CE-ED) method has been employed for the determination of six bioactive ingredients in traditional Chinese herbs, Herba cepbalanoplosis segeti and Herba cirsii japonici. The effects of several factors such as the acidity and concentration of running buffer, the separation voltage, the applied potential and the injection time on CE-ED were investigated. Under the optimum conditions, the six analytes could be well separated within 21 min in a 75 cm length capillary at the separation voltage of 15 kV in a 50 mmol L−1 borax running buffer (pH 8.4). A 300 μm diameter carbon disk electrode was used as the working electrode positioned carefully opposite the outlet of the capillary in a wall-jet configuration at potential of +950 mV (vs. SCE). Good linear relationship was established between peak current and concentration of analytes over two orders of magnitude with detection limits (S/N=3) ranged from 1.5×10−7 to 6.0×10−7 g mL−1 for all six analytes. This proposed method has been successfully applied for the analysis of traditional Chinese herbs after a relatively simple extraction procedure, further on, for the differentiation of these above two seemingly identical herbs based on their electropherograms or characteristic “electrochemical profiles”.

Keywords

Capillary electrophoresis Electrochemical detection Herba cepbalanoplosis segeti and Herba cirsii japonici 

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References

  1. Li LN (1998) Pure Appl Chem 70:547–554Google Scholar
  2. Committee of Jiangsu New Medicine College (1995) In: Encyclopedia of Traditional Chinese Medicine, Shanghai Science and Technology Press, Shanghai, pp. 242–244Google Scholar
  3. Committee of Jiangsu New Medicine College (1995) in: Encyclopedia of Traditional Chinese Medicine, Shanghai Science and Technology Press, Shanghai, pp.115–117Google Scholar
  4. Li Y, Wang GD (2003) Xinjiang J Trad Chinese Med 21:44–45Google Scholar
  5. Gu YC, Tu YY (1992) China J Chinese Materia Med 17:489–490Google Scholar
  6. Gu YC, Tu YY (1992) China J Chinese Materia Med 17:547–548, 576Google Scholar
  7. Miliauskasa G, Venskutonis PR, Beek T A van (2004) Food Chem 85:231–237CrossRefGoogle Scholar
  8. Brown JE, Khodr H, Hider RC, Rice-Evans CA (1998) Biochem J 330:1173–1178PubMedGoogle Scholar
  9. Cao GH, Emin S, Ronald LP (1997) Free Radical Bio Med 22:749–760CrossRefGoogle Scholar
  10. Kelly EH, Anthony RT, Dennis JB (2002) J Nutr Biochem 13:572–584CrossRefPubMedGoogle Scholar
  11. Catherine AR, Nicholas JM, George P (1996) Free Radical Bio Med 20:933–956CrossRefGoogle Scholar
  12. Chen YJ, Shiao MS, Hsu ML, Tsai TH, Wang SY (2001) J Agr Food Chem 49:5615–5619CrossRefGoogle Scholar
  13. Wang WY, Zhang TJ, Zhang K, Gong SX (2000) J Pharmaceut Pract 18:359Google Scholar
  14. Zhou WX, Tian Z (1994) Chinese J Pharmaceut Anal 14:39–40Google Scholar
  15. Zhang BS (1995) J Henan Trad Chinese Med Pharm 10:16–18Google Scholar
  16. Yang HY, Guo YW, Ye Q (1998) Northwest Pharmaceut J 13:155Google Scholar
  17. Zhou WX, Tian Z (1994) J Beijing Med Univ 26:309Google Scholar
  18. Zhang LX (2001) Gansu Chem Ind 15:112–114Google Scholar
  19. Chu QC, Qu WQ, Peng YY, Cao QH, Ye JN (2003) Chromatographia 58:67–71Google Scholar
  20. Cao YH, Chu QC, Ye JN (2002) Anal Bioanal Chem 374:294–299CrossRefPubMedGoogle Scholar

Copyright information

© Friedr. Vieweg&Sohn Verlagsgesellschaft mbH 2004

Authors and Affiliations

  • Q. C. Chu
    • 1
  • Y. H. Cao
    • 2
  • Y. Y. Peng
    • 1
    • 3
  • L. Fu
    • 1
  • J. N. Ye
    • 1
  1. 1.Department of ChemistryEast China Normal UniversityShanghaiP.R. China
  2. 2.School of Chemical and Material EngineeringSouthern Yangtze UniversityWuxiP.R. China
  3. 3.Department of ChemistryQuanzhou Normal CollegeFujianP.R. China

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