Chromatographia

, Volume 60, Issue 3–4, pp 207–211 | Cite as

A New Bioautographic Screening Method for the Detection of Estrogenic Compounds

  • M. B. Müller
  • C. Dausend
  • C. Weins
  • F. H. Frimmel
Article

Abstract

The yeast estrogen screen was introduced as biological detection method for high performance thin layer chromatography. Yeast cells were grown directly on HPTLC plates, where in the presence of estrogenic substances the production of the enzyme β-galactosidase was induced. For the natural ligand 17 β-estradiol, sensitivity could be improved by a factor of 20 using the fluorogenic substrate 4-methylumbelliferyl β-D-galactopyranoside instead of the chromogenic substrate chlorophenol red-β-D-galactopyranoside. The fluorescence intensity of “estrogenic” zones was measured using a commercial TLC-Scanner. A nearly full dose-response curve was obtained for 17 β-estradiol masses between 2.75 and 550 pg, or concentrations of 2.75 to 550 μg L−1.

Keywords

Thin layer chromatography Direct bioautography Yeast estrogen screen β-Galactosidase Endocrine disrupters 

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Copyright information

© Friedr. Vieweg&Sohn Verlagsgesellschaft mbH 2004

Authors and Affiliations

  • M. B. Müller
    • 1
  • C. Dausend
    • 1
  • C. Weins
    • 2
  • F. H. Frimmel
    • 1
  1. 1.Engler-Bunte-InstitutBereich WasserchemieKarlsruheGermany
  2. 2.Landesanstalt für Umweltschutz des Saarlandes (LfU)SaarbrückenGermany

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