Skip to main content
SpringerLink
Log in

A Rapid Densitometric Method for the Quantification of Luteolin in Medicinal Plants Using HPTLC

  • Published:
Chromatographia Aims and scope Submit manuscript

Abstract

Luteolin, a flavonoid, is reported to occur widely in many medicinal plants. It has been shown to have important biological activities. We report sensitive HPTLC method for the quantification of luteolin from plant material. The method was validated for precision, repeatability and accuracy. The method was found to be precise with RSDs for intraday in the range of 0.77–1.29% and inter−day in the range of 1.02–2.08%. Instrumental precision and repeatability of the method were found to be 0.39 and 0.57 (%CV). Accuracy of the method was checked by recovery study conducted at two different levels and the average percentage recovery was found to be 100.92%. The method was used for quantification of luteolin in three important herbal drugs viz. fruit of Cuminum cyminum, whole plant of Bacopa monnieri, flower of Achillea millefolium. The proposed HPTLC method for the quantification of luteolin was found to be simple, precise, specific, sensitive and accurate and can be used for quality control of raw materials.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Zhou ZD, Wang LY, Wang P (1979) Chin Herb Med Commun 10:35

    Google Scholar 

  • Annul Cooperation Group, Preliminary experimental study of Aruga decumbens Thunb against chronic bronchitis (1973) Chin Herb Med 2:18

    Google Scholar 

  • Amellal M, Bronner C, Barincon F, Haag M, Anton R, Landy Y (1994) Planta Med 118

  • Kawasaki M, Toyda M, Teshima R, Sawada J, Hayashi T, Arishawa M, Shimzu M (1994) Shokuhin Eiseigaku Zasshi (J Food Hyg SocJapan) 35 (5):497

    Google Scholar 

  • Kim HK, Cheon BS, Kim SY, Kim HP (1999) Biochem Pharmacol 58:769

    Article  Google Scholar 

  • Shimol K, Saka N, Kaji K, Nazawa R, Kinae N (2000) Biofactors 12 (1–4):181

  • Zarzueio A, Jimenez I, Gamez MJ, Utrilla P (1996) Life Sciences 58 (25):2311

    Google Scholar 

  • Nagao M, Morita N, Yahagi, T; Shimizu M, Kuroyanagi M, Fukuoka M (1981) Environ Mutagen 3(4):401

    CAS  PubMed  Google Scholar 

  • Rice−Evans CA, Miller NJ, Paganga G (1997) Trends in Plant Sciences 2:152

    Article  Google Scholar 

  • Miller NJ (1998) In Natural Antioxidants and Food Quality in Atherosclerosis and Cancer Prevention. Kumpulainen JT, Salonan JT (eds) The Royal Society of Chemistry, London p. 256–259

  • Kuppusamy UR, Das NP (1991) Experientia 47:1196

    CAS  PubMed  Google Scholar 

  • Cai Q, Rahn RO, Zhang, QR (1997) Cancer Lett 119:98

    Article  Google Scholar 

  • Elangovan V, Ramamoorthy N, Balasubramanian S, Sekar N, Govindasamy S (1994) Indian J Pharmacol 26(4):266

    CAS  Google Scholar 

  • Edenharder R, Rauscher R, Platt KL, (1997) Mutation Res 379:21

    Article  CAS  PubMed  Google Scholar 

  • Fecka I, Cisowski W (2002) J Planar Chromatogr 15:429

    CAS  Google Scholar 

  • Ku Y-R, Chen C-Y, Ho L-K, Lini J-H, Chang Y-S (2002) J Food Drug Anal 10 (3):139

    Google Scholar 

  • Areias FM, Valentao P, Andrade PB, Ferreres F, Seabra RM (2001) Food Chem 73:307

    Article  CAS  Google Scholar 

  • Liu FF, Catharina YWA, Heinze TM, Rankin JD, Berger RD, Freeman JP, Lay Jr JO (2000) J Chromatogr A 888:85

    Article  CAS  PubMed  Google Scholar 

  • Liu C-S, Song YS, Zhang K-J, Ryu J-C, Kim M, Zhou T-U (1995) J Pharm Biomed Analysis 13:1409

    Article  CAS  Google Scholar 

  • Natsume M, Osakabe N, Yamagishi M, Takizawa T, Nakamum T, Miyatake H, Hatano T, Yosbida TT (2000) Biosci Biotechnol Biochem 64:2581

    CAS  PubMed  Google Scholar 

  • Ferran S-R, Olga J, Isidre C, Cristina A-L, Maria I-P, Rosa L-R (2003) J Mass Spectrum 38:35

    Article  Google Scholar 

  • Chopra RN, Nayar SL, Chopra IC (1956) In: Glossary of indian medicinal plants. Council of Scientific and Industrial Research, New Delhi, 84, 45

  • The wealth of India, raw materials, (2000) Council of Scientific and Industrial Research, New Delhi, Cl-Cy, Vol 2:257

  • Sambaiah K, Srinivasan K (1989) Indian J Biochem Biophys 254

  • The Ayurvedic pharmacopoeia of India, (1999) Govt of India, Ministry of Health & Family Welfare, Department of Indian System of Medicine, New Delhi, India, Part-I, Vol-II:25

  • Nadkarni KM (1954) In: The Indian materia medica, Popular Book Depot, Bombay, 3rd ed. Vol. I:1315

  • Sivarajan VV, Balachandran I (eds.) (1994) BRAHMI (Mal. BRAHMI). In: Ayurvedic drugs and their plant sources, Oxford & IBH Publishing Co. Pvt. Ltd. New Delhi, 97–99

  • Jain P, Kulshreshtha DK (1993) Phytochemistry 33(2):449

    Article  CAS  Google Scholar 

  • Rastogi S, Pal R, Kulshreshtha DK (1994) Phytochemistry 36(1):133

    Article  CAS  PubMed  Google Scholar 

  • Rastogi S, Kulshreshtha DK (1998) Indian J Chem 38B:353

    Google Scholar 

  • Garai S, Mahato SB, Ohtani K, Yamasaki K (1996a) Phytochemistry 42(3):815

  • Garai S, Mahato SB, Ohtani K, Yamasaki K (1996b) Phytochemistry 43(2):447

  • Mahato SB, Garai S, Chakravarty AK (2000) Phytochemistry 53:711

    Article  CAS  PubMed  Google Scholar 

  • Basu NK, Walia JS (1944) Indian J Pharm 6:91

    Google Scholar 

  • Basu NK, Pabrai PR (1947) Quart J Pharm Pharmacol 20:137

    CAS  Google Scholar 

  • Schulte KE, Ruecker G, El-Kersch M (1972) Phytochemistry 11(8):2649

    Article  CAS  Google Scholar 

  • Proliac A, Chabaud A, Raynaud AJ (1991) Pharm Acta Helv 66:153

    CAS  Google Scholar 

  • Chatterji N, Rastogi RP, Dhar ML (1963) Indian J Chem 1:212

    Google Scholar 

  • Leon C, Daniel J, FR. Demande FR, 2,578,422 (Cl. A61K35/78), 12 Sep 1986, Appl 85/3,266, 05 Mar 1985, 19 pp

  • Tozyo T, Yoshimura Y, Sakurai K, Uchida N, Takeda Y, Nakai H, Ishii H (1994) Chem Pharm Bull 42,1096

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. B. V. Patel Pharmaceutical Education and Research Development (PERD) Centre, Thaltej-Gandhinagar Highway, Thaltej, Ahmedabad, – 380 054, India

    H. Srinivasa, M. S. Bagul, H. Padh & M. Rajani

Authors
  1. H. Srinivasa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. S. Bagul
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H. Padh
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. M. Rajani
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. Rajani.

Additional information

Revised: 1 October 2003 and 18 February 2004

Rights and permissions

Reprints and permissions

About this article

Cite this article

Srinivasa, H., Bagul, M., Padh, H. et al. A Rapid Densitometric Method for the Quantification of Luteolin in Medicinal Plants Using HPTLC. Chromatographia 60, 131–134 (2004). https://doi.org/10.1365/s10337-004-0302-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1365/s10337-004-0302-0

Keywords

Search

Navigation