Advertisement

Chromatographia

, Volume 60, Issue 1–2, pp 131–134 | Cite as

A Rapid Densitometric Method for the Quantification of Luteolin in Medicinal Plants Using HPTLC

Article

Abstract

Luteolin, a flavonoid, is reported to occur widely in many medicinal plants. It has been shown to have important biological activities. We report sensitive HPTLC method for the quantification of luteolin from plant material. The method was validated for precision, repeatability and accuracy. The method was found to be precise with RSDs for intraday in the range of 0.77–1.29% and inter−day in the range of 1.02–2.08%. Instrumental precision and repeatability of the method were found to be 0.39 and 0.57 (%CV). Accuracy of the method was checked by recovery study conducted at two different levels and the average percentage recovery was found to be 100.92%. The method was used for quantification of luteolin in three important herbal drugs viz. fruit of Cuminum cyminum, whole plant of Bacopa monnieri, flower of Achillea millefolium. The proposed HPTLC method for the quantification of luteolin was found to be simple, precise, specific, sensitive and accurate and can be used for quality control of raw materials.

Keywords

Thin layer chromatography Luteolin quantification Medicinal plants Cuminum cyminum Bacopa monnieri Achillea millefolium 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Zhou ZD, Wang LY, Wang P (1979) Chin Herb Med Commun 10:35Google Scholar
  2. Annul Cooperation Group, Preliminary experimental study of Aruga decumbens Thunb against chronic bronchitis (1973) Chin Herb Med 2:18Google Scholar
  3. Amellal M, Bronner C, Barincon F, Haag M, Anton R, Landy Y (1994) Planta Med 118Google Scholar
  4. Kawasaki M, Toyda M, Teshima R, Sawada J, Hayashi T, Arishawa M, Shimzu M (1994) Shokuhin Eiseigaku Zasshi (J Food Hyg SocJapan) 35 (5):497Google Scholar
  5. Kim HK, Cheon BS, Kim SY, Kim HP (1999) Biochem Pharmacol 58:769CrossRefGoogle Scholar
  6. Shimol K, Saka N, Kaji K, Nazawa R, Kinae N (2000) Biofactors 12 (1–4):181Google Scholar
  7. Zarzueio A, Jimenez I, Gamez MJ, Utrilla P (1996) Life Sciences 58 (25):2311Google Scholar
  8. Nagao M, Morita N, Yahagi, T; Shimizu M, Kuroyanagi M, Fukuoka M (1981) Environ Mutagen 3(4):401PubMedGoogle Scholar
  9. Rice−Evans CA, Miller NJ, Paganga G (1997) Trends in Plant Sciences 2:152CrossRefGoogle Scholar
  10. Miller NJ (1998) In Natural Antioxidants and Food Quality in Atherosclerosis and Cancer Prevention. Kumpulainen JT, Salonan JT (eds) The Royal Society of Chemistry, London p. 256–259Google Scholar
  11. Kuppusamy UR, Das NP (1991) Experientia 47:1196PubMedGoogle Scholar
  12. Cai Q, Rahn RO, Zhang, QR (1997) Cancer Lett 119:98CrossRefGoogle Scholar
  13. Elangovan V, Ramamoorthy N, Balasubramanian S, Sekar N, Govindasamy S (1994) Indian J Pharmacol 26(4):266Google Scholar
  14. Edenharder R, Rauscher R, Platt KL, (1997) Mutation Res 379:21CrossRefPubMedGoogle Scholar
  15. Fecka I, Cisowski W (2002) J Planar Chromatogr 15:429Google Scholar
  16. Ku Y-R, Chen C-Y, Ho L-K, Lini J-H, Chang Y-S (2002) J Food Drug Anal 10 (3):139Google Scholar
  17. Areias FM, Valentao P, Andrade PB, Ferreres F, Seabra RM (2001) Food Chem 73:307CrossRefGoogle Scholar
  18. Liu FF, Catharina YWA, Heinze TM, Rankin JD, Berger RD, Freeman JP, Lay Jr JO (2000) J Chromatogr A 888:85CrossRefPubMedGoogle Scholar
  19. Liu C-S, Song YS, Zhang K-J, Ryu J-C, Kim M, Zhou T-U (1995) J Pharm Biomed Analysis 13:1409CrossRefGoogle Scholar
  20. Natsume M, Osakabe N, Yamagishi M, Takizawa T, Nakamum T, Miyatake H, Hatano T, Yosbida TT (2000) Biosci Biotechnol Biochem 64:2581PubMedGoogle Scholar
  21. Ferran S-R, Olga J, Isidre C, Cristina A-L, Maria I-P, Rosa L-R (2003) J Mass Spectrum 38:35CrossRefGoogle Scholar
  22. Chopra RN, Nayar SL, Chopra IC (1956) In: Glossary of indian medicinal plants. Council of Scientific and Industrial Research, New Delhi, 84, 45Google Scholar
  23. The wealth of India, raw materials, (2000) Council of Scientific and Industrial Research, New Delhi, Cl-Cy, Vol 2:257Google Scholar
  24. Sambaiah K, Srinivasan K (1989) Indian J Biochem Biophys 254Google Scholar
  25. The Ayurvedic pharmacopoeia of India, (1999) Govt of India, Ministry of Health & Family Welfare, Department of Indian System of Medicine, New Delhi, India, Part-I, Vol-II:25Google Scholar
  26. Nadkarni KM (1954) In: The Indian materia medica, Popular Book Depot, Bombay, 3rd ed. Vol. I:1315Google Scholar
  27. Sivarajan VV, Balachandran I (eds.) (1994) BRAHMI (Mal. BRAHMI). In: Ayurvedic drugs and their plant sources, Oxford & IBH Publishing Co. Pvt. Ltd. New Delhi, 97–99Google Scholar
  28. Jain P, Kulshreshtha DK (1993) Phytochemistry 33(2):449CrossRefGoogle Scholar
  29. Rastogi S, Pal R, Kulshreshtha DK (1994) Phytochemistry 36(1):133CrossRefPubMedGoogle Scholar
  30. Rastogi S, Kulshreshtha DK (1998) Indian J Chem 38B:353Google Scholar
  31. Garai S, Mahato SB, Ohtani K, Yamasaki K (1996a) Phytochemistry 42(3):815Google Scholar
  32. Garai S, Mahato SB, Ohtani K, Yamasaki K (1996b) Phytochemistry 43(2):447Google Scholar
  33. Mahato SB, Garai S, Chakravarty AK (2000) Phytochemistry 53:711CrossRefPubMedGoogle Scholar
  34. Basu NK, Walia JS (1944) Indian J Pharm 6:91Google Scholar
  35. Basu NK, Pabrai PR (1947) Quart J Pharm Pharmacol 20:137Google Scholar
  36. Schulte KE, Ruecker G, El-Kersch M (1972) Phytochemistry 11(8):2649CrossRefGoogle Scholar
  37. Proliac A, Chabaud A, Raynaud AJ (1991) Pharm Acta Helv 66:153Google Scholar
  38. Chatterji N, Rastogi RP, Dhar ML (1963) Indian J Chem 1:212Google Scholar
  39. Leon C, Daniel J, FR. Demande FR, 2,578,422 (Cl. A61K35/78), 12 Sep 1986, Appl 85/3,266, 05 Mar 1985, 19 ppGoogle Scholar
  40. Tozyo T, Yoshimura Y, Sakurai K, Uchida N, Takeda Y, Nakai H, Ishii H (1994) Chem Pharm Bull 42,1096Google Scholar

Copyright information

© Friedr. Vieweg&Sohn Verlagsgesellschaft mbH 2004

Authors and Affiliations

  1. 1.B. V. Patel Pharmaceutical Education and Research Development (PERD) CentreIndia

Personalised recommendations