, Volume 59, Supplement 2, pp S165–S170 | Cite as

Enhanced Detection of Sulphur and Phospho-rous Containing Compounds in HPLC-Induc-tively Coupled Plasma Mass Spectrometry Using Chemical Resolution via Hexapole-Based Reaction with Oxygen

  • C. J. Smith
  • I. D. Wilson
  • L. Weidolf
  • F. Abou-Shakra
  • M. Thomsen


The sensitive detection of sulphur and phosphorous-containing compounds using HPLC-ICPMS is difficult due to the high background caused by polyatomic interferences. One potential solution to the problem of polyatomic interferences for S and P is to react the latter with oxygen in the collision hexapole to separate them from interferences eg; (O2 on S and NOH on P). This has the effect of moving the detection mass from 31 and 32 to 47 and 48 m/z for P and S respectively. This region is clear from interferences thereby providing an increase in the sensitivity of detection for the analytes of approximately 50–100 fold. For omeprazole, a model S containing compound, a limit of detection (LOD) of 800 pg on column was achieved, an increase of ca. 100 fold in sensitivity. Similarly in the case of ZD6126 a phosphorous-containing pro-drug, an increase in sensitivity of 50 times was observed with an LOD of 1 ng on column.


Column liquid chromatography Inductively coupled plasma mass spectrometry Phosphorous-containing pro-drugs Sulphur-containing drugs 


  1. Wilson and Wilsons Comprehensive Analytical Chemistry. Volume XXXIVGoogle Scholar
  2. Beauchemin D (2002) Anal Chem 74:2873–2894CrossRefPubMedGoogle Scholar
  3. Galettis P, Carr JL (1999) J Anal At Spectrom 14:953–956Google Scholar
  4. Smith CJ, Wilson ID, Abou-Shakra F, Payne R, Parry TC, Sinclair P, Roberts DW (2003) Anal Chem 75:1463–1469CrossRefPubMedGoogle Scholar
  5. Smith CJ, Wilson ID, Abou-Shakra F, Payne R, Grisedale H. Long A, Roberts D, Malone M (2002) Chromatographia 55:S151–S155Google Scholar
  6. Nicholson JK, Lindon JC, Scarfe G, Wilson ID (2000) Analyst 125:235–236CrossRefPubMedGoogle Scholar
  7. Abou-Shakra F, Sage AB, Wilson ID, Casrro-Perez J, Lindon JC, Nicholson JK, Scarfe GB (2002) Chromatographia 55:9–13Google Scholar
  8. Corcoran O, Nicholson JK, Lenz EM, Abou-Shakra F, Wilson ID (2000) Rapid Comm Mass Spectrom 14:1–8CrossRefGoogle Scholar
  9. Nicholson JK, Lindon JC, Scarfe G, Wilson ID, Abou-Shakra F, Sage AB, Castro-Perez J (2001) Anal Chem 73:1491–1494CrossRefPubMedGoogle Scholar
  10. Evans HE, Wolff J, Eckers C (2001) Anal Chem 73:4722–4728Google Scholar
  11. Axelsson B-O, Jörnten-Karlsson M, Michelsen P, Abou-shakra F (2001) Rapid Commun. Mass Spectrom. 15:375–385Google Scholar
  12. Tanner SD, Baranov VI (1999) J Am Soc Mass Spectrom 10:1083–1094CrossRefGoogle Scholar
  13. Bandura RD, Baranov VI, Tanner SD (2002) Anal Chem 74:1497–1502CrossRefPubMedGoogle Scholar
  14. Jiang SJ, Houk RS (1988) Spectrochim Acta 43B:405–411Google Scholar

Copyright information

© Friedr. Vieweg&Sohn Verlagsgesellschaft mbH 2004

Authors and Affiliations

  • C. J. Smith
    • 1
  • I. D. Wilson
    • 1
  • L. Weidolf
    • 2
  • F. Abou-Shakra
    • 3
  • M. Thomsen
    • 4
  1. 1.Department of Drug Metabolism and PharmacokineticsAstraZenecaUK
  2. 2.Department of Drug Metabolism and Pharmacokinetics and Bioanalytical ChemistryMölndalSweden
  3. 3.GV InstrumentsUK
  4. 4.PerkinElmer InstrumentsUK

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