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Chromatographia

, Volume 59, Supplement 2, pp S165–S170 | Cite as

Enhanced Detection of Sulphur and Phospho-rous Containing Compounds in HPLC-Induc-tively Coupled Plasma Mass Spectrometry Using Chemical Resolution via Hexapole-Based Reaction with Oxygen

  • C. J. Smith
  • I. D. Wilson
  • L. Weidolf
  • F. Abou-Shakra
  • M. Thomsen
Article
  • 82 Downloads

Abstract

The sensitive detection of sulphur and phosphorous-containing compounds using HPLC-ICPMS is difficult due to the high background caused by polyatomic interferences. One potential solution to the problem of polyatomic interferences for S and P is to react the latter with oxygen in the collision hexapole to separate them from interferences eg; (O2 on S and NOH on P). This has the effect of moving the detection mass from 31 and 32 to 47 and 48 m/z for P and S respectively. This region is clear from interferences thereby providing an increase in the sensitivity of detection for the analytes of approximately 50–100 fold. For omeprazole, a model S containing compound, a limit of detection (LOD) of 800 pg on column was achieved, an increase of ca. 100 fold in sensitivity. Similarly in the case of ZD6126 a phosphorous-containing pro-drug, an increase in sensitivity of 50 times was observed with an LOD of 1 ng on column.

Keywords

Column liquid chromatography Inductively coupled plasma mass spectrometry Phosphorous-containing pro-drugs Sulphur-containing drugs 

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Copyright information

© Friedr. Vieweg&Sohn Verlagsgesellschaft mbH 2004

Authors and Affiliations

  • C. J. Smith
    • 1
  • I. D. Wilson
    • 1
  • L. Weidolf
    • 2
  • F. Abou-Shakra
    • 3
  • M. Thomsen
    • 4
  1. 1.Department of Drug Metabolism and PharmacokineticsAstraZenecaUK
  2. 2.Department of Drug Metabolism and Pharmacokinetics and Bioanalytical ChemistryMölndalSweden
  3. 3.GV InstrumentsUK
  4. 4.PerkinElmer InstrumentsUK

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