Chromatographia

, Volume 58, Issue 11–12, pp 717–722 | Cite as

Micellar Electrokinetic Chromatography as a Valuable Technique for the Quality Control of Drugs Based on the Combination of Sulfamethoxazole with Trimethoprim

  • J. J. Berzas Nevado
  • G. Castañeda Peñalvo
  • F. J. Guzmán Bernardo
Article
  • 33 Downloads

Summary

A micellar electrokinetic chromatographic method is presented for the determination of sulfanilic acid and sulphanilamide as degradation products of sulfamethoxazole and of 3,4,5-trimethoxybenzoic acid as degradation product of trimetoprim, by using 15 mM phosphate buffer (pH 12.6), 20 mM sodium dodecylsulfate and 5% (v/v) acetonitrile as electrolyte. The separation was carried out at 25 kV and 25 °C in a fused silica capillary (60.2 cm × 75 μm i.d.). Under these conditions, the run time was less than 10 min and the limits of quantification were 2.0–4.2 mg L−1. The reproducibility in-a-day of the assay was excellent because the relative standard deviations of the peak areas were always under 3%. The method was applied to spiked samples of a pharmaceutical commercial preparation containing the sulfamethoxazole-trimethoprim combination, and the recoveries obtained were close to 100% for both sulfanilic acid and sulfanilamide.

Key Words

Column liquid chromatography Micellar electrokinetic chromatography Quality control Sulfamethoxazole Degradation products of sulfamethoxazole 

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Copyright information

© Friedr. Vieweg&Sohn Verlagsgesellschaft mbH 2003

Authors and Affiliations

  • J. J. Berzas Nevado
    • 1
  • G. Castañeda Peñalvo
    • 1
  • F. J. Guzmán Bernardo
    • 1
  1. 1.Departamento de Química Analítica y Tecnología de Alimentos, Facultad de Ciencias QuímicasUniversidad de Castilla-La ManchaCiudad RealSpain

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