Separation and Purification of Annexin B1, a Novel Ca2+-Dependent Phospholipid Binding Protein, Expressed in Escherichia coli by Ion-Exchange Chromatography
Annexin B1 has been cloned in Escherichia coli (E. coli) K802 in previous works. After temperature shift induction the protein was expressed as soluble form. Then a separation method for annexin B1 from E. coli cells by ion-exchange chromatography has been developed and validated. The method exhibited a good reproducibility and is easy to operate. It has shown that annexin B1 isolated by chromatography had high anticoagulant activity.
Key WordsColumn liquid chromatography Ion-exchange chromatography Annexin B1 Escherichia coli
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