Chromatographia

, Volume 58, Issue 11–12, pp 713–716 | Cite as

Separation and Purification of Annexin B1, a Novel Ca2+-Dependent Phospholipid Binding Protein, Expressed in Escherichia coli by Ion-Exchange Chromatography

  • Yi Zhang
  • Yingjun Guo
  • Yuanjian Gao
  • Shu Zhang
  • Hongli Yan
  • Shuhan Sun
Article
  • 86 Downloads

Summary

Annexin B1 has been cloned in Escherichia coli (E. coli) K802 in previous works. After temperature shift induction the protein was expressed as soluble form. Then a separation method for annexin B1 from E. coli cells by ion-exchange chromatography has been developed and validated. The method exhibited a good reproducibility and is easy to operate. It has shown that annexin B1 isolated by chromatography had high anticoagulant activity.

Key Words

Column liquid chromatography Ion-exchange chromatography Annexin B1 Escherichia coli 

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Copyright information

© Friedr. Vieweg&Sohn Verlagsgesellschaft mbH 2003

Authors and Affiliations

  • Yi Zhang
    • 1
  • Yingjun Guo
    • 1
  • Yuanjian Gao
    • 1
  • Shu Zhang
    • 1
  • Hongli Yan
    • 1
  • Shuhan Sun
    • 1
  1. 1.Department of Medical GeneticsThe Second Military Medical UniversityShanghaiP.R. China

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