Rapid Determination of Salicylic Acid in Plant Materials by Gas Chromatography–Mass Spectrometry
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Salicylic acid (SA) is a signaling compound in plants such as tobacco, cucumber, and tomato which can induce systemic acquired resistance. In the work discussed in this paper a simple, rapid, and sensitive method was developed for determination of salicylic acid in plant tissues by gas chromatography–mass spectrometry (GC–MS). SA from tomato leaves extracted with 9:1 (v/v) methanol–chloroform was derivatized by use of bis(trimethylsilyl)trifluoroacetamide (BSTFA) under the optimum reaction conditions (120 °C, 60 min). Quantitative analysis by GC–MS was performed in selected ion monitoring (SIM) mode using an internal standard. Procedures for sample preparation and reaction conditions were optimized. Analysis was completed within 2 h. A sensitivity of 10 ng g−1 fresh weight and a relative standard deviation less than 5.0% for SA in tomato leaves were achieved. The method could be used for investigation of SA in plant tissues to monitor fast responses of plant defense.
Key WordsGas chromatography–mass spectrometry Derivatization with BSTFA Salicylic acid Plant tissue
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