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Enantiomeric Resolution of Benzoxazolinonic Aminoalcohols, Potential Adrenergic Ligands, by LC and CE

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Abstract

Benzoxazolinone amino alcohols are chiral derivatives that carry one asymmetric center. Both capillary electrophoresis and high-performance liquid chromatography (LC) techniques were used to separate enantiomers with high resolution and thus permitted the determination of enantiomeric excess. Highly sulfated and anionic cyclodextrins were used as chiral selectors under CE conditions. Amylose and cellulose chiral stationary phases were used for LC for the enantioseparation of five racemic compounds. Separations were carried out on a Chiralpak AS column with various polar organic modifiers. The HPLC method appeared more to be more sensitive and was subsequently validated in terms of limits of detection and quantification. LC detection was based on UV and evaporating light scattering.

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Authors and Affiliations

  1. Laboratoire de Chimie Analytique-EA 4034, Faculté des Sciences Pharmaceutiques et Biologiques, Université de Lille 2, BP 83, 3, rue du Pr. Laguesse, 59006, Lille Cedex, France

    Emmanuelle Lipka, Claude Vaccher & Jean-Paul Bonte

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  1. Emmanuelle Lipka
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  2. Claude Vaccher
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  3. Jean-Paul Bonte
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Correspondence to Claude Vaccher.

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Lipka, E., Vaccher, C. & Bonte, JP. Enantiomeric Resolution of Benzoxazolinonic Aminoalcohols, Potential Adrenergic Ligands, by LC and CE. Chroma 69, 465–472 (2009). https://doi.org/10.1365/s10337-008-0940-8

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