Chinese Science Bulletin

, Volume 50, Issue 24, pp 2896–2900 | Cite as

Detection and analysis system for hybridization images of lab-in-a-tube microarray

  • Quanjun Liu
  • Qin Zhou
  • Yunfei Bai
  • Qinyu Ge
  • Zuhong Lu
Articles
  • 15 Downloads

Abstract

A lab-in-a-tube microarray system is developed for sample inspection and signal detection by fabricating a flat transparent window cap of the Eppendorf tube. The oligonucleotide microarray is immobilized on the inner surface of the cap. A small vessel is placed in an Eppendorf tube for storing hybridization solutions. With the microarray system, the full biochemical processes, including gene fragment amplification, fluorescence labeling, hybridization, and fluorescence detection, have been performed in the sealed tube without opening the cap. The images are obtained from a fluorescence microscope and captured by a CCD, and the data are transported to a computer through the universal serial bus (USB). After noise reduction, signal intensity is determined from hybridization image and the presence of gene fragments is identified. The final data output includes sample information, process steps, and hybridization results. A lab-in-a-tube microarray system for detecting ten respiratory viruses at a single detection is designed. High detection throughput and accuracy have been demonstrated with the system.

Keywords

lab-in-a-tube microarray respiration virus detection image processing 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Gerhold, D., Rushmore, T., Caskey, C. T., DNA chips: Promising toys have become powerful tools, Techniques, 1993, 3: 168–173.Google Scholar
  2. 2.
    Khan, J., Bitter, N. L., Chen Y. D. et al., DNA microarray technology: The anticipated impact on the study of human diease, Biochemica et Biophysica Acta, 1999, 1423: 17–28.Google Scholar
  3. 3.
    Oliphant, A., Barker, D. L., Stuelpnagel, J. R. et al., BeadArray (TM) technology: Enabling an accurate, cost-effective approach to high throughput genotyping, Biotechniques, 2002, 32: S56-S61.Google Scholar
  4. 4.
    D. Shalon, S., Smith, J., Brown, P. O., DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization, Genome Res., 1996, 6: 639–645.CrossRefGoogle Scholar
  5. 5.
    Guo, Z., Guilfoyle, R. A., Thiel, A. J., Direct fluorescence analysis of genetic polymorphisms by hybridization with oligonucleotide arrays on glass supports, Nucleic Acids Res., 1994, 22: 5456–5465.PubMedCrossRefGoogle Scholar
  6. 6.
    Heller, R. A., Schena, M., Chai, A. et al., Discovery and analysis of inflammatory disease-related genes using cDNA microarrays, Pro. Natl. Acd. Sci. USA, 1997, 94: 2150–2155.CrossRefGoogle Scholar
  7. 7.
    Lashkari, D. A., DeRisi, J. L., McCusker, J. H. et al., Yeast microarrays for genome wide parallel genetic and gene expression analysis, Pro. Natl. Acd. Sci. USA, 1997, 94: 13057–13062.CrossRefGoogle Scholar
  8. 8.
    Sinclair, M. B., Timlin, J. A., Haaland, D. M. et al., Design, construction, characterization, and application of a hyperspectral microarray scanner, Applied Optics, 2004, 43: 2079–2088.PubMedCrossRefGoogle Scholar
  9. 9.
    Drosten, C., Gunther, S., Preiser, W. et al., Identification of a novel coronavirus in patients with severe acute respiratory syndrome, N. Engl. J. Med., 2003, 348: 1967–1976.PubMedCrossRefGoogle Scholar
  10. 10.
    Yam, W. C., Chan, K. H., Poon, L. L. M. et al., Evaluation of reverse transcription-PCR assays for rapid diagnosis of severe acute respiratory syndrome associated with a novel coronavirus, J. Clin. Microbiol., 2003, 41: 4521–4524.PubMedCrossRefGoogle Scholar
  11. 11.
    Fodor, S. P., Read, J. L., Pirrung, M. C., Stryer, L., Lu, A. T, Solas, D., Light-directed, spatially addressable parallel chemical synthesis, Science, 1991, 251: 767–773.PubMedCrossRefGoogle Scholar
  12. 12.
    Fodor, S. P., Rava, R. P., Huang, X. C. et al., Multiplexed biochemical assays with biological chips, Nature, 1993, 364: 555–556.PubMedCrossRefGoogle Scholar

Copyright information

© Science in China Press 2005

Authors and Affiliations

  • Quanjun Liu
    • 1
  • Qin Zhou
    • 1
  • Yunfei Bai
    • 1
  • Qinyu Ge
    • 1
  • Zuhong Lu
    • 1
  1. 1.State Key Laboratory of BioelectronicsSoutheast UniversityNanjingChina

Personalised recommendations