The 5′ untranslated region of fruA mRNA in Myxococcus xanthus positively regulates the expression of its own gene
- 9 Downloads
Myxococcus xanthus provides an excellent model organism for studying the mechanism of multicellular morphogenesis. The mRNA for FruA, a transcription factor essential for the development of M. xanthus, contains a very long 5′-UTR consisting of 235 nucleotides. Using lacZ as a reporter gene, two fruA-lacZ translational fusions retaining or lacking the fruA 5′-UTR were constructed and separately integrated at phage Mx8 attachment site (attB) in M. xanthus chromosome. Deletion in 5′-UTR between nucleotides from +4 to +220 abolished fruA-lacZ expression during development, indicating that the 5′-UTR is essential for the induction of fruA. Prediction of the RNA secondary structure of 5′-UTR shows that this region could form an extremely stable three-helix junction structure, which might be a binding site for a regulatory protein or contain a cis-acting element(s) to control fruA expression. Thus, the 5′-UTR of fruA mRNA positively regulates the expression of its own gene.
KeywordsMyxococcus xanthus fruA 5′-UTR development regulation
Unable to display preview. Download preview PDF.
- 1.Dworkin, M., Recent advances in the social and developmental biology of the myxobacteria, Microbiol. Rev., 1996, 60: 70–102.Google Scholar
- 3.Zhou, L., Li, Y. Z., Li, J., Multicellular morphogenesis in Myxobacteria and its molecular regulation, Prog. Biochem. Biophys., 1999, 26(6): 544–547.Google Scholar
- 4.Ogawa, M., Fujitani, S., Mao, X. et al., FruA, a putative transcription factor essential for the development of Myxococcus xanthus, Mol. Microbiol., 1996, 22: 7 57–767.Google Scholar
- 11.Magrini, V., Creighton, C., White, D. et al., The aadA gene of plasmid R100 confers resistance to spectinomycin and streptomycin in Myxococcus xanthus, J. Bacteriol., 1998, 180: 6757–6760.Google Scholar
- 13.Tojo, N., Sanmiya, K., Sugawara, H. et al., Integration of bacteriophage Mx8 into the Myxococcus xanthus chromosome causes a structural alteration at the C-terminal region of the IntP protein, J. Bacteriol., 1996, 178: 4004–4011.Google Scholar
- 14.Li, S. F., Shimkets L. J., Site-specific integration and expression of a developmental promoter in Myxococcus xanthus, J Bacteriol, 1988, 170: 5552–5556Google Scholar
- 15.Kil, K. S., Brown, G. L., Downard, J. S., A segment of Myxococcus xanthus ops DNA functions as an upstream activation site for tps gene transcription, J. Bacteriol., 1990, 172: 3081–3088.Google Scholar