Advertisement

Science in China Series C: Life Sciences

, Volume 48, Issue 6, pp 641–647 | Cite as

Discovery and identification of anti-U1-A snRNP antibody in lung cancer

  • Zhang Lijuan 
  • Jifu Liu
  • Hao Zhang
  • Shanshan Wu
  • Lingyun Huang
  • Dacheng He
  • Xueyuan Xiao
Article
  • 16 Downloads

Abstract

There are multiple reports of autoimmune response in patients with lung cancer. To investigate whether a novel autoantibody is present in patients with lung cancer and evaluate its clinical diagnostic and prognostic value, sera from 10 patients with lung cancer and 10 normal individuals were analyzed using immunofluorescence and Western blotting. It was found that one serum sample from the patients with squamous carcinoma gave a fine speckled pattern staining in nucleus and had a high titer antinuclear autoantibody which could recognize 31 kD of nuclear protein isolated from both cancer cells and normal cells. The same patient’s serum was further used to immunoprecipitate the target antigen. The protein bands were excised from the SDS-PAGE gels and were analyzed with a Qstar Pulser I Quadrupole time-flight mass spectrometer, and the 31 kD target antigen was identified as U1-AsnRNP. To test the prevalence of anti-U1-AsnRNP antibody, sera from 93 patients including 36 squmaous carcinomas (SCC), 26 adenocarcinomas (Ad), and 31 small cell carcinomas (SCLC) were screened by Western blotting. The results demonstrated that anti-U1-A snRNP antibody was present in 50% of SCC sera, 26.9% of Ad sera and 54.8% of SCLC sera. In this paper, we report for the first time that anti-U1-AsnRNP antibody could be detected in the patients with lung cancer.

Keywords

lung cancer autoantibody U1-A snRNP 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Soussi, T., p53 antibodies in the sera of patients with various types of cancer: A review, Cancer Res., 2000, 60: 1777–1788.PubMedGoogle Scholar
  2. 2.
    Tomkiel, J. E., Alansari, H., Tang, N. et al., Autoimmunity to the Mr. 32000 subunit of replication protein A in breast cancer, Clin. Cancer Res., 2002, 8: 752–758.PubMedGoogle Scholar
  3. 3.
    Felix, F. M., Pamela, J., Vande, V. et al., Antinuclear antibodies as potential markers of lung cancer, Clin. Cancer Res., 1999, 5: 1393–1400.Google Scholar
  4. 4.
    Zeng, C., He, D., Berget, S. M. et al., Nuclear-Mitotic apparatus protein: Structural protein interface between the nucleoskeleton and RNA splicing, Proc. Natl. Acad. Sci. USA, 1994, 91: 1505–1509.PubMedCrossRefGoogle Scholar
  5. 5.
    Xiao, X. Y., Liu, D. H., Tang, Y. et al., Development of proteomic patterns for detecting lung cancer, Dis. Markers, 2003, 19(1): 33–39.PubMedGoogle Scholar
  6. 6.
    Chen, P., Xie, J. Y., Liang, S. P., Identification of protein spots in sliver-stained two-dimensional gels by MALDI-TOF mass peptide map analysis, Acta Biochim. Biophys. Sin., 2000, 32: 387–391.PubMedGoogle Scholar
  7. 7.
    Adam, B. L., Qu, Y., Davis, J. W. et al., Serum protein fingerprinting coupled with a pattern-matching algorithm distinguishes prostate cancer from benign prostate hyperplasia and healthy men, Cancer Res., 2002, 62: 3609–3614.PubMedGoogle Scholar
  8. 8.
    Petricoin, III E. F., Ardekani, A. M., Hitt, B. A. et al., Use of proteomic patterns in serum to identify ovarian cancer, The Lancet, 2002, 359: 572–577.CrossRefGoogle Scholar
  9. 9.
    Xiao, X. Y., Zhao, X., Liu, J. K. et al., Discovery of laryngeal carcinoma by serum proteomic pattern analysis, Sci. China, 2004, 47(3): 219–223.Google Scholar
  10. 10.
    Félix, F. M., Pamela, J., Vande, V. et al., Antinuclear antibodies as potential markers of lung cancer, Clin. Cancer Res., 1999, 5: 1393–1400.Google Scholar
  11. 11.
    Kubo, M., Ihn, H., Kuwana, M. et al., Anti-U5 snRNP antibody as a possible serological marker for scleroderma-polymyositis overlap, Rhrumatology, 2002, 41: 531–534.CrossRefGoogle Scholar

Copyright information

© Science in China Press 2005

Authors and Affiliations

  • Zhang Lijuan 
    • 1
  • Jifu Liu
    • 2
  • Hao Zhang
    • 1
  • Shanshan Wu
    • 2
  • Lingyun Huang
    • 1
  • Dacheng He
    • 1
  • Xueyuan Xiao
    • 1
  1. 1.Key Laboratory of Cell Proliferation and Regulation of Ministry of EducationBeijing Normal UniversityBeijingChina
  2. 2.Department of Thoracic SurgeryGeneral Hospital of Beijing Unit, PLABeijingChina

Personalised recommendations